雄性生殖细胞冷冻保存研究进展 |
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引用本文: | 王丽[综述] 姜宏[审校]. 雄性生殖细胞冷冻保存研究进展[J]. 国外医学:计划生育分册, 2013, 0(6): 478-480 |
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作者姓名: | 王丽[综述] 姜宏[审校] |
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作者单位: | 安徽医科大学解放军临床学院生殖医学中心,合肥230031 |
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摘 要: | 随着单精子及精子细胞卵胞浆内显微受精技术的发展,雄性生殖细胞冷冻保存技术已成为辅助生殖技术的重要内容,为男性因素不育患者的临床助孕提供了物质保障基础。目前,雄性生殖细胞冷冻方法包括程序化冷冻和玻璃化冷冻;冷冻保护剂可有效降低细胞的冷冻损伤,根据其特性分为渗透性保护剂和非渗透性保护剂两大类;冷冻载体中冷冻环、冷冻叶片等应用较广泛。雄性生殖细胞冷冻的效果与冷冻方法、冷冻保护剂和冷冻载体等密切相关。
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关 键 词: | 低温保存 精液保存 精子 精细胞 精子注射,细胞质内 玻璃化冷冻 冷冻载体 冷冻保护剂 |
Progress on the Cryopreservation of Male Germ Cell |
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Affiliation: | WANG Li,JIANG Hong. Reproductive Medical Center, Clinical College of People's Liberation Army ,A nhui Medical University ,Hefei 230031, China |
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Abstract: | Following the development of intracytoplasmic sperm and haploid spermatid injection techniques,the cryopreservation technology of male germ cell, as important part of assisted reproductive technology (ART), has played a key role in the ART treatment for the couples with male infertility. Freezing methods of male germ cells include the programmed cryopreservation and vitrification freezing. Cryoprotectant , usually classified as permeable and impermeable cryoprotective agents,could minimize the cell injuries during the freeze-thaw. Various frozen carriers such as cryoloop and cryoleaf are widely used in cell cryopreservation. In this paper,the freezing method,carrier,cryoprotectants and frozen effect of male germ cell were reviewed. |
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Keywords: | Cryopreservation Semen preservation Spermatozoa Spermatids Sperm injections,intracytoplasmic Vitrification Frozen carrier Cryoprotectant |
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