急性坏死性胰腺炎大鼠血清消褪素的表达变化及意义

目的观察急性坏死性胰腺炎（ANP）大鼠血清消褪素D1（resolvin D1，RvD1）和白细胞介素-6（IL-6）、IL-10的变化情况，探讨RvD1在急性坏死性胰腺炎发病机制中的作用。方法70只SD大鼠随机分为ANP模型组（n=35）和假手术组（r／=35）。经十二指肠乳头逆行胆胰管注射4％牛磺胆酸钠来制备大鼠ANP模型。大鼠分别于造模后3h、6h、12h、24h、48h处死，测定大鼠血清淀粉酶，光镜下进行胰腺病理学观察及评分，应用酶联免疫吸附测定（ELISA）法检测血清RvD1和IL-6、IL-10的变化情况。结果ANP组大鼠血清淀粉酶从造模后3h开始升高，6h达峰值（8244．00±2949．83）u／L，各时点血清淀粉酶显著高于假手术组（P〈0．05）；ANP组胰腺病理损伤及评分于造模后3h～24h随时间延长逐渐加重，24h评分为（11．91±1．312）分，均较假手术组高（P〈0．05）。ANP组血清RvD1水平于造模后3h～24h随时间延长逐渐升高，24h达峰值（39．48±15．28）pg／mL，较假手术组显著升高（P〈0．05），并与血清IL-6水平呈正相关（r=0．362，P〈0．05）；在造模后6h、12h、24h，ANP组的血清IL-6水平均较假手术组高（P〈0．05），并随时间延长逐渐升高，于24h达峰值（1293．26±428．12）pg／mL；在造模后3h、24h和48h，ANP组的血清IL-10水平均较假手术组显著升高（P〈0．05）。结论血清Rv的变化可能与急性胰腺炎（AP）严重程度有关，在AP中起抗炎及促进炎性反应消退的作用。

Expression and significance of serum resolvin D1 in rats with acute necrotizing pancreatitis

Objective To observe the expression of serum resolvin D1 （RvD1）, IL6 and IL-10 in rats with acute necrotizing pancreatitis （ANP）, and investigate the role of RvD1 in nosogenesis of ANP. Methods Seventy SD rats were randomly divided into the ANP group （n = 35） and the sham-operation group （n = 35）. In the ANP group, the rats were induced by the retrograde injection of bilio-pancreatic duct with 4% sodium taurocholate and sacrificed after taking the blood sample and the pancreas 3 h, 6 h, 12 h, 24 h and 48 h after cessation of sodium taurocholate injection. The pathologic changes of pancreas were observed and graded under microscope, the level of serum amylase was detected with biochemical analyzer, and the level of serum RvD1 , IL-6, IL-10 were evaluated using ELISA. Results The serum amylase level began to increase at 3 h after sodium taurocholate injection and reached the peak value at 6 h （8244.00 ± 2949.83） U/L], and were significantly higher than those of the sham operation group all the time （P〈0.05）. Pancreatic injuries of the ANP group aggravated with time, the pathologic score of 24 h was （11. 91 ± 1. 312）, and were significantly higher than those of the sham-operation group （P〈 0.05）. The level of serum RvD1 in the ANP group increased gradually with time （3 h-24 h）, reached the peak level at 24 h （39.48 ± 15.28） pg/ml, was significantly higher than that of the sham-operation group （P〈0.05）, and positively related to the level of serum IL-6 （r=0. 362, P〈0.05）. The level of serum IL-6 in the ANP group was significantly higher than that of the sham-operation group at 6 h, 12 h and 24 h （P〈0.05）, increased gradually with time, and reached the peak level at 24 h （1293.26 ± 428. 12） pg/mL. The level of serum IL-10 in the ANP group was significantly higher than that of the sham-operation group at 3 h, 24 h, 48 h （P〈0.05）. Conclusion The level of serum resolvin might be related to the severity of acute pancreatitis （AP）, and might play the role of anti-inflammatory in AP.