乙型肝炎病毒表面抗原弱反应性样本的确认与分析

目的对酶联免疫吸附试验（ELISA）定性的乙型肝炎病毒表面抗原（HBsAg）弱反应性样本进行确认及分析。方法收集经ELISA检测HBsAg的S／CO值为0．7～3．0的样本共613例，以中和试验确认，同时进行微粒子酶免疫方法（MEIA）检测，不确定病例继续随访。结果经确认共41例与ELISA判定结果不符，假阳性率4．9％，假阴性率10．7％；其中S／CO值0．9～1．2范围产生的假阴性率或假阳性率最高。中和试验和MEIA法检测结果基本一致，仅4例不符。结论ELISA检测HBsAg呈弱反应性，尤其是“灰区”结果应予以确认，中和试验和MEIA都是有效的方法，个别病例还需参考病史长期随访。

Confirmation and analysis of weak reactive hepatitis B surface antigen

Objective To confirm weak reactive HBsAg detected by enzyme-linked immunosorbent assay （ELISA）. Methods Neutralization test and MEIA are used to detected HBsAg of specimens whose S/CO value was from 0.7 to 3.0 by ELISA, while the cases that can＇t be confirmed will be follow-- up studied. Results 41 cases showed different results by neutralization test and ELISA, and the false positive rate was 4.9% ; the false negative rate was 10.7%, and the specimens of S/CO value from 0.9 to 1.2 showed highest false rate. There were only 4 cases had different results by neutralization test and MEIA. Conclusion Weak reactive HBsAg tested by ELISA,especially the grey zone results should be confirmed by neutralization test. MEIA is also effective,and follow--up study is needed for some special cases.