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| SCF与bFGF联合诱导脐血CD133~+细胞分化及基因表达的变化 | |
| 引用本文： | 张静,朱敬松,李倩如,杜英,杨波,李国喜,胡祥. SCF与bFGF联合诱导脐血CD133~+细胞分化及基因表达的变化[J]. 中国实验血液学杂志, 2010, 18(1): 146-150 |
| 作者姓名： | 张静 朱敬松 李倩如 杜英 杨波 李国喜 胡祥 |
| 作者单位： | 1. 郑州大学基础医学院微生物学与免疫学教研室,河南郑州,450001 2. 郑州大学第二附属医院放射科,河南郑州,450003 3. 郑州大学第一附属医院神经外科,河南郑州,450052 4. 深圳市北科细胞工程研究所,广东深圳,518057 |
| 基金项目： | 广东省科技计划项目,编号2007B031500015 |
| 摘 要： | 本研究探讨干细胞因子(SCF)与碱性成纤维细胞生长因子(bFGF)体外联合诱导脐血CD133+细胞分化及基因表达的变化,认识脐血CD133+细胞的生物学特性,为体外诱导分化和临床应用打下基础。利用MiniMACS免疫磁珠法从脐静脉血单个核细胞中分离出CD133+细胞,用含干细胞因子(SCF)、bFGF、B27的DMEM/F12细胞营养液培养10-15天,提取培养前后细胞总RNA,利用Oligo GEArray芯片和GEArray表达分析软件进行相关基因表达的芯片数据分析。结果表明:在所检测的263个基因中,发现21个基因培养后表达比培养前显著上调,7个基因表达显著下调。这些基因主要涉及干细胞特异性标志、细胞周期、干细胞分化标志以及与干细胞相关的信号转导、细胞因子及其受体等。结论:SCF和bFGF通过一些特定基因的变化影响脐血CD133+细胞增殖分化,这一结果有助于从基因水平理解SCF与bFGF联合作用对CD133+细胞增殖分化的影响,有利于细胞因子诱导的CD133+细胞在临床上的应用。 |
| 关 键 词： | 干细胞因子 碱性成纤维细胞生长因子 CD133细胞 基因芯片 脐血 |
Changes of Differentiation and Gene Expression of CD133+Cells in Human Umbilical Cord Blood Induced by SCF and bFGF |
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| ZHANG Jing,ZHU Jing-Song,LI Qian-Ru,DU Ying,YANG Bo,LI Guo-Xi,HU Xiang. Changes of Differentiation and Gene Expression of CD133+Cells in Human Umbilical Cord Blood Induced by SCF and bFGF[J]. Journal of experimental hematology, 2010, 18(1): 146-150 | |
| Authors: | ZHANG Jing ZHU Jing-Song LI Qian-Ru DU Ying YANG Bo LI Guo-Xi HU Xiang |
| Affiliation: | ( Department of Microbiology and Immunology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, Henan Province, China ; 1 Department of Radiology, The Second Affiliated Hospital, Zhengzhou University , Zhengzhou 450003, Henan Prov- ince, China; 2Department of Neurosurgery , The First Affiliated Hospital ,Zhengzhou University ,Zhengzhou 450052 ,Henan Province, China; 3 Shenzhen Beike Cell Engineering Institute, Shenzhen 519057, Guangdong Province, China) |
| Abstract: | This study was aimed to investigate the changes of differentiation and gene expression of CD133+ cells in human umbilical cord blood induced by stem cell factor(SCF) and basic fibroblast growth factor(bFGF) in vitro,and to explore the biological characteristics of CD133+ cells so as to provide experimental basis for potential use in regenerative medicine.The human umbilical cord blood CD133+ cells were isolated from umbilical cord blood and purified by MACS magnetic selection.The CD133+ cells were cultured ... |
| Keywords: | stem cell factor bFGF CD133 cell oligonucleotide microarray human umbilical cord blood |
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