HPLC法同时测定升血小板胶囊中丹皮酚、靛玉红的含量

目的建立升血小板胶囊中丹皮酚、靛玉红的含量测定方法。方法采用高效液相色谱法,色谱条件：色谱柱：Agilent E Clipse XDS-C18 4.6×250 mm 5 m;流动相：甲醇-水（70：30）;检测波长为293nm,流速mL·min-1。结果 制剂中其他药材对丹皮酚、靛玉红无干扰;丹皮酚在0.2252~2.252μg之间有良好线性关系（r=1.0000）,平均回收率为103.0,（n=6,RSD=1.7%）;靛玉红在0.0282~0.282μg之间有良好线性关系（r=0.9999）,平均回收率为104.9,（n=6,RSD=2.3%）。结论 本方法简便,快速,灵敏,专属性强,重现性好,可作为本药品中丹皮酚、靛玉红的含量测定方法。

A Method of HPLC to Detect the Content of Paeonol and Indirubin in Shengxuexiaoban Capsules

Objective To establish a method of HPLC to detect the content of paeonol and indirubin in Shengxuexi- aoban capsules. Methods Agilent EClipse XDS-C18 column （4.6mm×250mm, 5μm） was used. The mobile phase was methanol-water （70：15）. The detection wavelength was 293 nm and the flow rate was 1.0 mL min-1. Results The detection of paeonol and indirubin＇s content was not influenced. The calibration curve was linear in the range of 0.2252-2.252 μg for paeonol （r=l.0000）, the average recovery（n=6） was 103.0, and relative standard deviation was 1.7%.The calibration curve was linear in the range of 0.0282-0.282 μg for indirubin （r=0.9999）, the average recovery（n=6） was 104.9, and relative standard deviation was 2.3%. Conclusion The method is simple, rapid, sensitive, highly specific and well repeatable, it can be used to detect the content of paeonol and indirubin in Shengxuexiaoban capsules.