HPLC-ELSD测定复方血栓通胶囊中三七皂苷R1、人参皂苷Rg1及Rb1的含量

目的：建立高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法同时测定复方血栓通胶囊中三七皂苷R1、人参皂苷Rg1及Rb1的含量。方法：采用蒸发光散射检测器（ELSD）,色谱柱为Diamonsil C18（4.6mm×250mm,5μm）;乙腈（A）-水（B）为流动相,梯度洗脱（0min→5min→20min,A：20%→25%→45%）;流速1.0mL/min;柱温25℃;ELSD参数：载气流量2.0L/min,漂移管温度70℃。结果：三七皂苷R1在0.3-1.5μg之间呈良好的线性关系（r=0.9994）,平均回收率（n=6）为98.8%,RSD为1.1%;人参皂苷Rg1在1.5-7.5μg之间呈良好的线性关系（r=0.9995）,平均回收率（n=6）为98.2%,RSD为1.8%;人参皂苷Rb1在1.5-7.5μg之间呈良好的线性关系（r=0.9999）,平均回收率（n=6）为97.6%,RSD为1.3%。结论：该法精密度、重复性良好,操作简单、快捷,结果准确,可用于复方血栓通胶囊的质量控制。

Simultaneous Assay of Notoginsenoside R1, Ginsenoside Rgl and Rbl in Fufang Xueshuantong Capsules by HPLC -ELSD

Objective：To establish simultaneous assay of the contents of notoginsenoside R1, ginsenoside Rgl and Rbl in Fufang Xueshuantong capsules by HPLC -ELSD. Methods： The column was packed with a DiamonsilC18 （4.6mm × 250mm, 5μm） stationary phase. The mobile phase consisted of acetonitrile（A） - water （B）, eluted in gradient mode（0 min→15min→20min,A：20%→25% →45% ） with flow rate of 1.0mL/min. Column temperature was set at 25℃. The temperature of driftube was 70℃ and the air flow rate was 2.0L/ rain. Results ： The linear ranges of notoginsenoside R1, ginsenoside Rgl and Rbl were 0.3 - 1.5 μg （ r = 0. 9994 ） , 1.5 ~ 7.5μg （ r = 0.9995 ） and 1.5 - 7.5μg（ r = 0. 9999 ） respectively ; the average recoveries （ n = 6） were 98.8% ,98.2% and 97.6% ,with the RSD of 1.1%, 1.8% and 1.3% respectively. Conclusion： The method is rapid, simple and accurate. It can be used for quality evaluation of Fufang Xueshuantong capsules.