采用生物反应器体外构建大面积组织的接种技术

目的　采用培养袋生物反应器进行大面积支架材料细胞接种技术的研究。方法　以人体成纤维细胞和PET无纺布支架材料为模型，考察水平摇床的转速和起始细胞悬液密度对细胞接种动力学、细胞接种率、支架中细胞密度以及细胞分布的影响。结果　在实验条件范围内，成纤维细胞接种大面积PET支架材料的过程基本符合一级反应动力学；低细胞悬液密度接种时，接种率和支架中细胞密度随转速的增加而下降，高细胞悬液密度接种时则反之；细胞分布均匀度都随着转速的增加而下降，起始细胞悬液密度对细胞分布影响较小。结论　采用培养袋生物反应器进行大面积支架材料的细胞接种是可行的，研究结果为进一步优化适合大面积支架材料接种的方法奠定基础。

Cell Seeding for large-area Tissue Construction in Bioreactor

Objective To research on cell seeding for large-area tissue construction in a bag bioreactor. Method A tissue engineered 3-D model system was developed by using human fibroblasts and porous polyethylene terephthalate (PET) polymer scaffolds. The effects of shaking speed and initial cell concentration in suspension on cell seeding kinetic, cell seeding efficiency, initial seeding density and cell distribution within the scaffolds were studied. Result Under the experimental conditions, the process of human fibroblasts seeding to large-area PET generally followed the first order reaction kinetics. And at low initial cell concentration, the seeding efficiency and initial seeding density decreased with the increasing shaking speed, while at high initial cell concentration the results were reverse. The cell distributing uniformity all decreased with shaking speed increased for all different cell concentrations, but there was no significant difference in uniformity between different initial cell concentrations under the same shaking intenslty. Conclusions The horizontal shake seeding method used in a bag bioreactor for large-area scaffold was approved feasible, which provides basic foundation for further optimizing cell seeding method for such large-area tissue construction.