| 紫外分光光度法测定射干中总异黄酮的含量 |
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| 引用本文： | 李国信,杨明,康廷国,田原.紫外分光光度法测定射干中总异黄酮的含量[J].辽宁中医杂志,2008,35(3):428-429. |
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| 作者姓名： | 李国信 杨明 康廷国 田原 |
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| 作者单位： | 1. 辽宁中医药大学,辽宁,沈阳,110032
2. 辽宁中医药大学附属医院中医药实验中心,辽宁,沈阳,110032 |
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| 摘 要： | 目的:建立射干药材中总异黄酮的含量测定方法,为控制药材的内在质量提供依据。方法:采用紫外分光光度法对射干药材中的总异黄酮含量测定,测定波长为266nm。结果:射干苷浓度在1~9μg/mL范围内与吸收度呈良好的线性关系(r=0.9998,n=5),平均加样回收率为98.52%(RSD=1.9%,n=5)。结论:该方法准确,简便,重现性好,可用作测定射干中总异黄酮的含量的方法。
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| 关 键 词： | 紫外分光光度法 射干 射干苷 含量 |
| 文章编号： | 1000-1719(2008)03-0428-02 |
| 修稿时间： | 2007年11月8日 |
Determination of total isoflavones in Rhizolna Belamcandae. by UV spectrophotometry |
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| LI Guo-xin,YANG Ming,KANG Ting-guo,TIAN Yuan.Determination of total isoflavones in Rhizolna Belamcandae. by UV spectrophotometry[J].Liaoning Journal of Traditional Chinese Medicine,2008,35(3):428-429. |
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| Authors: | LI Guo-xin YANG Ming KANG Ting-guo TIAN Yuan |
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| Abstract: | Objective:To establish a method for the assaying of total isoflavonoes in the ultrasonic wave's extracts of Rhizoma Belamcandae and to provide basis for the study of its internal quality.Method:The contents of total flavonoes in Belamcanda were determined by UV spectrophotometry with a detection wavelength at 266nm.Results:Good linear relationship with absorbability occurred when the concentration range of tectoridin was 1～9μg/mL(r=0.9998,n=5)and its average recovery was 98.52%(RSD=1.9%,n=5).Conclusion:The method is simple,quick and accurate,of the testing samples,and can be used to determine the content of total flavonoes in Rhizoma Belamcandae. |
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| Keywords: | UV spectrophotometry Belamcandae tectoridin Content |
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