日本血吸虫生殖细胞染色体制备及G带带型分析

目的制备日本血吸虫雄虫生殖细胞减数分裂时期的染色体,分析其染色体核型及G带特征。方法向感染血吸虫的小鼠腹腔注射秋水仙素,5h后处死小鼠收集成虫,快速切下30条雄虫睾丸部分,按常规气干法制备染色体,一部分玻片直接Giemsa染色,观察染色体核型。另一部分玻片用胰酶消化法制备G带,初步分析G带特征。结果日本血吸虫性腺细胞染色体数目为n=8,核型公式为n=2m+3Sm+2St+性染色体;各对染色体显示出特征性的G带。结论本研究方法可成功制备日本血吸虫生殖细胞减数分裂时期染色体,获得了较理想的染色体核型图像和G带带纹。

Analysis of karyotype and G band type on chromosome of the reproductive cells from Schistosoma japonicum

To analyze the characters of karyotype and G band type in the meiotic mitosis phase of reproductive cells of Schisosoma japonicum,the infected mice were injected with colchicine(4 μg/g)intra-peritoneally,killed 4-6 hrs later and then the testis of mice was sliced off,from which the chromosome slides were prepared by routine air-drying method.Some of these slides were stained with Giemsa attain to observe the karyotype,and the other slides were treated with trypsin digestion method for the preliminary study on character of the G band type.It was demonstrated that the chromosome number of the reproductive cells was found to be 8.The karyotype formula was 2m + 3Sm + 2St + sex chromosome and distinctive G bands could be identified in every pairs of chromosomes.It is evident that the method we used can prepare chromosome on meiotic mitosis phase of reproductive cells of S japonicum with characteristic G band stripe.