miR-218-1-3p对非小细胞肺癌细胞侵袭和迁移影响

目的 非小细胞肺癌(non-small cell lung cancer,NSCLC)因其侵袭性而导致预后不良,miR-218可对多种肿瘤的进展起到抑制作用.本研究探讨miR-218-1-3p对NSCLC A549细胞侵袭和迁移影响.方法 使用LipofectamineTM 2000 Reagent将miR-218-1-3p mimic转染入NSCLC A549细胞中,实时定量聚合酶链反应(real-time PCR,RT-qPCR)检测各组细胞中miR-218-1-3p的表达,Transwell小室法测定其侵袭及迁移能力,荧光定量PCR检测细胞迁移侵袭相关指标基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)、MMP-7、MMP-9、Rho A、Rho B和Rho C的变化.结果 同对照组相比,上调mir-218-1-3p明显抑制了A549细胞的侵袭(t=4.028,P=0.016)和迁移(t=8.911,P=0.001),其侵袭和迁移的抑制率分别为37.8%和53.6%.MMP-7降低至对照组的(0.68±0.19)倍,t=2.931,P=0.043;MMP-9降低至对照组的(0.58±0.15)倍,t=4.875,P=0.080.结论 miR-218-1-3p能够抑制NSCLC A549细胞的侵袭和迁移.

Effects of miR-218-1-3p on cell migration and invasion in non-small cell lung cancer

OBJECTIVE Non small-cell lung cancer leads to unfavourable prognosis because of invasion,miR-218 can inhibit the progression of multiple tumors.This research was to investigate the effect of miR-218-1-3p on the invasion and migration of A549 cells in non small cell lung cancer.METHODS miR-218-1-3p was transfected into non small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent,the expression of miR-218-3p was detected by real-time PCR. The invasion and migration were assayed by Transwell method.The changes of indicators related to cell migration and in-vasion,MMP-2,MMP-7,MMP-9,Rho,A,Rho B,Rho C were detected by fluorescence quantitative PCR. RESULTS Comparing with control group,the cell invasion (t=4.028,P =0.016)and migration (t=8.911,P =0.001) of A549 were significantly inhibited by up-regulating mir-218-1-3p (P< 0.05),rating 37.8% and 53.6%.MMP-7 de-creased to (0.68±0.19)times of control group (t=2.931,P =0.043)and MMP-9 decreased to (0.58±0.15)times of control group (t=4.875,P =0.080).CONCLUSION miR-218-3p can inhibit the invasion and migration of non small cell lung cancer A549 cells.