宫颈鳞状细胞癌组织CD44V6与LYVE-1表达及临床意义

目的 宫颈癌是女性常见恶性肿瘤之一,其发病率和年轻化趋势逐年上升,深入研究其癌变机制,对宫颈癌的预后有重要意义.本研究旨在探讨宫颈鳞状细胞癌组织中CD44V6和 LYVE-1表达的相关性及临床意义.方法 采用免疫组化SP法检测2010-03-01-2014-02-28山东大学齐鲁医院宫颈鳞状细胞癌(cervicalsquamous cell carcinoma,CSC)44例(CSC组)、正常宫颈上皮(normal cervical epithelium,NCE)18例(NCE组)及宫颈鳞状上皮内病变(squamous intraepithelial lesion,SIL)21例(SIL组)组织中CD44V6和LYVE-1表达情况,统计分析其表达水平与临床病理因素的相关性.结果 宫颈鳞状细胞癌组织中CD44V6蛋白主要表达于细胞膜上;LYVE-1蛋白主要定位于细胞质,在细胞膜上亦有表达.CSC组CD44V6阳性表达率为70.45%(31/44),显著高于SIL组的42.86%(9/21)和NCE组的11.11%(2/18),差异均有统计学意义,均P<0.05;SIL组CD44V6阳性表达率显著高于NCE组,P<0.05.CSC组 LYVE-1阳性表达率为77.27%(34/44),显著高于SIL组的52.38%(11/21)和NCE组的16.67%(3/18),差异均有统计学意义,均P<0.05;SIL组LYVE-1阳性表达率显著高于NCE组,P<0.05.宫颈鳞癌组织中CD44V6和LYVE-1表达与淋巴结转移相关,P<0.05.CD44V6表达与临床分期、组织学分级相关,P<0.05.CD44V6和LYVE-1表达水平呈正相关,r=0.722,P<0.001;生存分析结果显示,CD44V6及LYVE-1蛋白阳性组宫颈鳞癌患者生存率均低于阴性组.结论 CD44V6和LYVE-1在宫颈鳞状细胞癌发生发展中起重要作用,其过表达均与淋巴结转移有关,可作为评估预后和转移的生物学指标.

Expression of CD44V6 and LYVE-1 and its clinical significance in cervical squamous cell carcinoma

OBJECTIVE Cervical cancer was one of the most common female malignant tumor,and its incidence and the trend of youth rise year by year.Further study of the mechanisms of cancer was important for the prognosis of cervical cancer.The aim of the study was to investigate the correlation between CD44V6 and LYVE-1 and their clinical significance in cervical squamous cell carcinoma.METHODS The expression of CD44V6 and LYVE-1 was detected in 18 cases of normal cervical epithelium,21 cases of cervical squamous intraepithelial lesions, and 44 cases of cervical squamous carcinoma tissues were detected by immunohistochemical SP method,The expression of each protein and its clinicopathological significance in the tissue of carcinoma of cervix were investigated.RESULTS The CD44V6 protein in cervical squamous cell carcinoma tissue was mainly expressed in the cell membrane.LYVE-1 protein mainly located in cytoplasm,which also expressed in the cell membrane.The positive rate of CD44V6 and LYVE-1 in cervical squamous carcinoma was significantly higher than that of SIL group and NCE group,and the expression rate of each protein in SIL group was significantly higher than that of NCE group (P<0.05).Two kinds of molecular expression intensity was positively correlated with cervical squamous carcinoma lymphatic metastasis (P<0.05).CD44V6 expression was positively correlated with cervical squamous carcinoma clinical stage and histological grade (P<0.05).The expression levels of CD44V6 and LYVE-1 were positively correlated (r=0.722,P<0.001).Survival analysis showed that patients with positive CD44V6 and LYVE-1 expression had a significant poorer overall survival time compared with negative groups of patients (P<0.05).CONCLUSIONS Comparing the two kinds of molecular dyeing effects,CD44V6 and LYVE-1 coordinated the expression in cervical squamous carcinoma,might be new markers related to cervical squamous carcinoma metastasis and prognosis estimation.