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Detection and genetic characterization of canine parvoviruses and coronaviruses in southern Ireland
Authors:McElligott Susan  Collins P J  Sleator Roy D  Martella Vito  Decaro Nicola  Buonavoglia Canio  O'Shea Helen
Affiliation:(1) Department of Biological Sciences, Cork Institute of Technology, Rossa Avenue, Bishopstown, Cork, Ireland;(2) Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima Km3, 70010 Valenzano, Bari, Italy;
Abstract:Canine parvovirus (CPV) and canine coronavirus (CCoV) are considered the main pathogens responsible for acute gastroenteritis in dogs. From a collection of 250 samples, seven CPV strains and three CCoV strains were identified in symptomatic Irish dogs. Samples were screened for the viruses using polymerase chain reaction (PCR) and typed via DNA sequence analysis. Three CPV strains were characterized as CPV-2a, while four others were characterized as CPV-2b. To date, CPV-2c remains unreported in Ireland. Two CCoV strains were characterized as CCoV-II and one as CCoV-I. In the case of one sample, PH4/09/Ire, a mixed infection with CPV and CCoV was detected.
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