Improvements of in vitro cell-material adhesion measurements |
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| Authors: | Ronald D. Fletcher John K. Adamiak Maryann C. Labant John N. Abertson Jr |
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| Affiliation: | The Department of Microbiology, The University of Pittsburgh, Pittsburgh, Pennsylavania, and the U.S. Army Medical Research and Development Command, Ft. Detrick, Maryland. U.S.A |
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| Abstract: | The in vitro measurement of gingival cell-bacto agar substratum adhesive-bonding strength (γa) is described as it relates to cell- concentration, cell-substratum contact time and serum concentration. These three factors are critical in obtaining reprocible γa's for gingival cell-test substratum interactions.
Cell concentrations of less than 35 × 103 cells per cm2 gave low γa's (70–75 ergs/cm2) whereas 90–130 × 103 cells/cm2 resulted in γa's of 109–119 ergs/cm2, respectively. Maximum γa's (123 ergs/cm2) resulted after the cells had been in contact with the agar substratum for 24 hours. The adhesive-bonding strenth after eight housrs of contact time, however, was not statistically different from the 24 housr readings and has the advantage of decreasing the test duration. Serum, and important factor for optimum adhesion measurements, produced a peak γa at the 10 % concentration. |
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