慢性应激抑郁模型的建立及其评价

目的 探讨慢性轻度不可预见性的应激抑郁模型建立的可行性和此模型的有效性。方法 选用SD雄性大鼠制作慢性轻度不可预见性的应激抑郁模型。观察动物体重和摄食量的变化，记录动物敞箱试验(open—field test)和液体消耗试验。结果 与对照组比较，经历慢性轻度不可预见性应激的动物体重、摄食量显著下降；敞箱试验中的水平运动、垂直运动得分显著下降，中央格停留时间明显延长，清洁动作次数显著下降；液体消耗试验中糖水消耗和糖水偏爱百分比明显下降，而纯水消耗显著提高。结论 慢性轻度不可预见性的应激抑郁模型动物活动能力显著下降、兴趣丧失、快感缺乏与抑郁症临床表现中的精神运动性阻抑症状极为相似，且行为学改变可维持较长时间，是较理想可靠的抑郁动物模型。

The establishment and evaluation of chronic unpredictable mild stress depression model

Objective To study the validity, reliability and utility of the chronic unpredictable mild stress model of depression (CUMS). Methods Male Sprague Dawley rats were exposed to an unpredictable sequence of mild stressors. Body weight, daily food intake, open field test and fluid consumption were investigated during the whole procedure. Results Rats exposed to CUMS showed decreased body weight and food intake; reduced squares crossed, and rears and grooming and an increased central time stopped in open field test; a significant reduction of consumption of and preference for sucrose solutions and an increased pure water consumption. Conclusions The decreased locomotion, loss of interest and anhedonia founded in the CUMS animal model are similar to the feature of human depression. Furthermore, the behavioral changes produced by CUMS can maintain a long time. Thus, this animal model of depression seems indeed meet the three validity criteria necessary to validate an animal model of depression (construct validity, face validity, predictive validity).