哮喘豚鼠肺组织粒细胞-巨噬细胞集落刺激因子mRNA表达及雷公藤的影响

目的探讨粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）在哮喘嗜酸细胞（Ｅｏｓ）炎症中的作用。方法建立哮喘豚鼠实验模型，用雷公藤处理，观察肺组织Ｅｏｓ密度，斑点分子杂交检测支气管肺组织ＧＭ－ＣＳＦｍＲＮＡ含量。结果哮喘组肺组织Ｅｏｓ浸润密度增加，ＧＭ－ＣＳＦｍＲＮＡ表达水平增高，与对照组相比差异有显著性（Ｐ＜０．０５）。雷公藤处理后ｍＲＮＡ表达显著降低，但Ｅｏｓ浸润密度减少不明显。结论ＧＭ－ＣＳＦｍＲＮＡ表达增加可能是导致哮喘Ｅｏｓ气道炎症的原因。雷公藤能抑制ＧＭ－ＣＳＦｍＲＮＡ表达，可能具有哮喘抗炎治疗的潜在应用价值。

mRNA expression of granulocyte macrophage colony stimulating factor in airway tissues of asthma guinea pigs: Effect of tripholide

Objective To explore the role of granulocyte macrophage colony stimulating factor (GM CSF) in eosinophil inflammation of asthma airway. Methods Guinea pig models of asthma were established with aerosolized ovalbumin. A group of asthmatic guinea pigs were treated with injection of tripholide, density of eosinophil infilatration in airway tissues was observed under microscope and expression of GM CSF mRNA in airway tissues was detected with dot hybridization by Dig labeled cDNA probe. Results expression of GM CSF mRNA in asthmatic animals were higher markedly than that in the tripholide treated and the control groups. However, there was no statistically differences of density of eosinophils infiltration between the asthma and the tripholide treated groups. Conclusions GM CSF might participate in eosinophil inflammation in airway of asthmatic animals and tripholide might be of potential value in anti inflammation for asthma.