人骨髓间充质干细胞的培养及研究

目的　了解间充质干细胞(mesenchymal stem cell,MSC)的培养条件、生物特性及分化能力。方法　抽取健康成人骨髓,利用密度梯度离心分离培养人MSC,并用流式细胞仪检测细胞表面标记,体外诱导MSC向成骨细胞分化。结果　成功地进行了人MSC的原代和传代培养;MSC CD34、CD45、HLA DR、CD62p等为阴性,CD29、CD44、CD166、CD90 等为阳性;MSC能够分化为成骨细胞。结论　人骨髓MSC在体外有较强的扩增能力,并能够向成骨细胞分化。

Culture of Human Bone Marrow Mesenchymal Stem Cells

Objective To explore the culture conditions, biological characteristics and differentiation potentiality of mesenchymal stem cells(MSCs). Methods MSCs were collected and cultured ex vivo by density gradient centrifugation from human bone marrow and the expression of surface molecules and the ability of differentiation into osteoblast were examined by flow cytometric. Results MSCs could be cultured continually ex vivo; MSCs were negative on CD34, CD45, HLA-DR and CD62p, while positive on CD29, CD44, CD166 and CD90; MSCs had the potentiality of differentiating into osteoblasts. Conclusion The result suggested that MSCs derived from human bone marrow have strong reproducing ability ex vivo and can differentiate into osteoblasts in the certain condition.