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| P物质参与调控骨髓间充质干细胞源性成骨细胞的矿化 | |
| 引用本文： | 柳达,黄瑛,李彬,王广斌,许晓军,付勤,戴力扬. P物质参与调控骨髓间充质干细胞源性成骨细胞的矿化[J]. 解剖科学进展, 2010, 16(5) |
| 作者姓名： | 柳达 黄瑛 李彬 王广斌 许晓军 付勤 戴力扬 |
| 作者单位： | 1. 中国医科大学附属盛京医院骨科,辽宁,沈阳,110004 2. 中国医科大学附属盛京医院超声科,辽宁,沈阳,110004 3. 上海交通大学附属新华医院骨科,上海,200092 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的观察不同浓度、不同时间P物质(substance P,SP)对骨髓间充质干细胞源性成骨细胞矿化的影响,并用SP非特异性的阻滞剂和NK1受体特异性的阻滞剂进行干预,从而推测其作用细胞的靶受体。方法 10只雄性6周龄SD大鼠制作脊髓损伤模型。从模型的胫骨、股骨提取骨髓间充质干细胞(mesenchymal stemcells,MSCs)进行成骨细胞的诱导培养,用第二代细胞进行实验。施加不同浓度的SP,对照组不加任何处理因素,在不同的时间点观察骨髓MSCs源性成骨细胞的矿化。并用SP、Spantide(SP的抑制剂)、L703606(NK1受体的抑制剂)进行配伍作用,观察骨髓MSCs源性成骨细胞的矿化。结果不同浓度的SP对细胞的矿化有不同程度的抑制作用,10~(-8)M浓度的抑制作用最强,矿化结节面积较对照组平均减少了38.3%(P0.01),随时间的延长抑制作用越强(3W:-34.9%,P0.01)。细胞矿化的抑制可以被Spantide和L703606所逆转,与SP组比较两组矿化分别升高了45.3%和50.5%。结论 SP通过NK1受体对成骨细胞的矿化产生影响。SP抑制了成骨细胞的矿化,使成熟的成骨细胞减少,从而引起脊髓损伤后骨质疏松的发生。 |
| 关 键 词： | 脊髓损伤 骨质疏松 P物质 成骨细胞 矿化 SD大鼠 |
The effect of substance P on cell mineralization of osteoblasts derived from bone marrow mesenchyrmal stem cells in SD rats |
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| LIU Da,HUANG Ying,LI Bin,WANG Guang-bin,XU Xiao-jun,FU Qin,DAI Li-yang. The effect of substance P on cell mineralization of osteoblasts derived from bone marrow mesenchyrmal stem cells in SD rats[J]. Progress of Anatomical Sciences, 2010, 16(5) | |
| Authors: | LIU Da HUANG Ying LI Bin WANG Guang-bin XU Xiao-jun FU Qin DAI Li-yang |
| Affiliation: | LIU Da~(1*),HUANG Ying~2,LI Bin~1,WANG Guang-bin~1,XU Xiao-jun~1,FU Qin~1,DAI Li-yang~3 (1.Department of Orthopaedic Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,2.Department of Ultrasound,3.Department of Orthopaedic Surgery,Xinhua Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200092 China) |
| Abstract: | Objective To investigate the mineralization of osteoblasts after treated with SP in different density and different time course.Nonspecific and specific receptor blocking agents of SP were used to identify target receptors mediated SP action.Methods MSCs from tibiae and femurs in SCI rats were cultured under control mediums and induced to become osteoblasts.Cell mineralization was measured by alizarin red staining with different experimental conditions including various density,time,and blocking agents.Resu... |
| Keywords: | Spinal cord injury osteoporosis substance P osteoblasts mineralization SD rat |
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