纳达合剂对小鼠胃肠动力及胃肌电活动的影响

目的:观察纳达合剂对正常小鼠以及阿托品、盐酸麻黄碱诱导的胃肠动力障碍小鼠胃肠动力及胃肌电的调节作用。方法:50只ICR雄性小鼠,随机取分为正常组,纳达合剂低、中、高剂量组及吗丁啉组。另取120只ICR雄性小鼠,随机分为正常组20只、模型组100只。模型组小鼠腹腔注射阿托品或盐酸麻黄碱后,分别随机灌胃生理盐水、纳达合剂低、中、高剂量(38,25,25.5,12.75 g·kg~(-1))及吗丁啉进行干预。旨在观察纳达合剂对正常小鼠及模型小鼠胃肠推进率、胃残留率以及胃窦肌电慢波的影响。结果:与正常小鼠相比较,纳达合剂呈剂量依赖性显著提高正常小鼠胃肠推进率,减少其胃残留率,并对胃窦平滑肌慢波肌电的频率和振幅呈现促进效应。与模型组相比较,纳达合剂呈剂量依赖性提高小鼠胃肠推进率,减少其胃残留率,并对胃窦平滑肌慢波肌电频率和振幅有促进作用。结论:纳达合剂具有促进胃肠蠕动、调节胃肌电的作用。

Effect of Nada Mixture on Gastrointestinal Motility and Gastric Myoelecerical Activity in Mice

Objective: To observe the regulative effect of Nada mixture on the gastrointestinal motility and gastric myoelecerical activity in normal mice, as well as atropine and ephedrine-induced gastrointestinal motility disorder mice. Method: The 50 male ICR mice were randomly divided into normal group, Nada mixture low dose group, medium dose group, high dose group and domperidone group. Then, another 120 male ICR mice were randomly divided into normal group and model group. After injected with atropine or ephedrine hydrochloride in model group, mice were treated with normal saline, Nadal mixture of low, medium and high doses and domperidone to observe the effects of Nada mixture on gastrointestinal propulsion rate, gastric residual rate and slow wave of antral myoelectric in normal mice and model mice. Result: As compared with the normal mice, the model mice induced by atropine or ephedrine hydrochloride can significantly decrease gastrointestinal propulsion rate, increase the gastric residual rate, and suppress the frequency and amplitude of slow wave of gastric antrum smooth muscles. As compared with the model group, Nada mixture of each dose can significantly increase the gastrointestinal propulsion rate, promote the frequency and amplitude of slow wave of gastric antrum smooth muscles and reduce the gastric residual rate in a dose-dependent manner. Conclusion: Nada mixture can promote gastrointestinal motility and regulate gastric myoelecerical activity in mice.