川芎嗪对小鼠化学性肝损伤保护作用的实验研究

目的:研究川芎嗪(tetramethylpyrazine,TMP)对小鼠化学性肝损伤的作用及其可能机制。方法:采用在体和离体两种方法观察:在体以D-氨基半乳糖(D-galactosamine,D-Gal)诱导小鼠急性肝损伤模型,测定各组肝损伤小鼠血清谷丙转氨酶(glutamic pyruvic transaminase,GPT)、谷草转氨酶(glutamic-oxalacetic transaminase;GOT)活性,及肝组织丙二醛(malondialdehyde,MDA)、黄嘌呤氧化酶(xanthine oxidase,XOD)、谷胱甘肽过氧化物酶(glutathion peroxi-dase,GSH-PX)、一氧化氮(nitrogen monoxidum,NO)、一氧化氮合成酶(nitric oxide synthetase,NOS)、诱生型一氧化氮合酶(inducible nitric oxide synthase,iNOS)含量。体外实验采用小鼠离体肝细胞原代培养,并建立D-Gal诱导肝细胞坏死性损伤模型,检测TMP对其的影响。结果:TMP能显著降低小鼠血清中升高的GPT、GOT,降低XOD活力和过氧化物终产物MDA的含量。对氧化应激引起的肝脏GSH含量下降具有升高作用。TMP可以显著降低离体培养中染毒肝细胞中的GPT水平。结论:提示TMP对小鼠化学性肝损伤具有显著的保护作用。

Experimental Study of Tetramethylpyrazine Against Chemical Hepatic Injury in Mice

Objective:To investigate the effect of tetramethylpyrazine(TMP) on chemical liver injury in mice and its possible mechanisms.Methods:The acute hepatic injurymodel in mice was induced by D-Gal.We measured thechanges of activity of GPT and GOT in serum and MDA,XOD,GSH-PX,NO,NOS and iNOS content of liver tissue.The model of chemical liver injury in mice was prepared.In vitro,primary cultured mice hepatocyte was injured by D-Gal,and the level of GPT in medium was measured to study protective effect of TMP.Results:TMP could decrease the activities of GPT and GOT in serum and the MDA,XOD,NOS and iNOS content of liver tissue,and was able to increase the activities of GSH-PX content of liver tissue.In vitro,it is indicated that TMP had a notable protective effects on the hepatocyte injuredby D-Gal and could suppress the level of GPT.Conclusion:TMP had notable protective effect against acute liver injury in mice.