解毒活血配伍方药对载脂蛋白E基因敲除小鼠血清超敏C反应蛋白的影响

目的 观察解毒活血配伍方药对载脂蛋白E基因敲除［apolipoprotein E gene knock out，ApoE( / ) mice］小鼠血清超敏C反应蛋白（hs CRP）的影响。方法 13周龄110只ApoE( / )小鼠分为高脂饲料组（98只，给予高脂饲料），普通饲料组（12只，给予普通饲料），同时设13周龄C57 BL/6J小鼠作为正常对照组（12只，给予普通饲料）。19周后随机抽取高脂饲料ApoE( / )小鼠2只确认易损斑块形成后，剩余96只随机分为8组（模型组、解毒组、活血组、解毒活血配伍高、中、低剂量组、洛伐他汀组和血脂康组），每组12只。造模成功后每天给予药物干预，解毒组予虎杖提取物266 mg/kg；活血组予芎芍胶囊110 mg/kg；配伍高剂量组予虎杖提取物53.2 mg/kg，芎芍胶囊220 mg/kg；配伍中剂量组给予虎杖提取物26.6 mg/kg，芎芍胶囊110 mg/kg；配伍低剂量组给予虎杖提取物133 mg/kg，芎芍胶囊55 mg/kg；洛伐他汀组给予洛伐他汀3.3 mg/kg；血脂康组给予血脂康0.2 g/kg；以上药物根据剂量蒸馏水溶解，混匀后灌胃，每次0.4 mL。模型组、普通饲料组、C57BL/6J小鼠对照组均灌服生理盐水0.4 mL。用药17周后，下腔静脉取血，全自动酶标仪检测血清hs-CRP浓度。结果 模型组血清hs-CRP水平显著高于正常对照组和普饲组（P<0.05，P<0.01）；各给药组中，洛伐他汀组、解毒组和配伍高剂量组hs-CRP水平下降，与模型组比较，差异有统计学意义（P<0.01）；配伍高剂量组hs-CRP水平低于洛伐他汀组、血脂康组、单纯解毒或活血组及配伍中、低剂量组；解毒组hs-CRP水平低于活血组（P<0.01）。结论 解毒活血配伍方药可降低ApoE( / )小鼠血清hs-CRP水平。

Effect of Assorted Use of Chinese Drugs for Detoxifying and Activating Blood Circulation on Serum High Sensitive C-reactive Protein in Apolipoprotein E Gene Knock-out Mice

OBJECTIVE: To observe the regulatory effect of assorted use of Chinese drugs for detoxifying and activating blood circulation on serum high sensitive C-reactive protein (hs-CRP) in apolipoprotein E gene knock-out ApoE (-/-)] mice. METHODS: ApoE (-/-) mice of 13-week old were devided into two groups, 12 in Group A fed with common forage and 98 in Group B with high lipid forage. Besides, 12 C57 BL/6J mice, 13-week old, were set as Group C fed with common forage. After being fed for 19 weeks, and the formation of vulnerable plaque had been confirmed in 2 mice of Group B, the other 96 mice were sub-divided into 8 groups, 12 in each group. Except that 0.4 mL normal saline was infused to the Group B1 as well as Group A and C, the other 7 sub-groups of Group B were respectively medicated with various drugs as follows: B2, Polydatin (PD, an extract from giant knotweed rhizome for detoxicating) 26. 6 mg/kg; B3, Xiongshao Capsule (XS, a Chinese herbal preparation for activating blood circulation), 110 mg/kg; B4, PD 53.2 mg/kg + XS 220 mg/kg; B5, PD 26. 6 mg/kg + XS 110 mg/kg; B6, PD 13.3 mg/kg + XS 55 mg/kg; B7, Lovastatin 3.3 mg/kg; and B8, Xuezhikang (XZK, a Chinese patent drug) 0.2 g/kg, all were administered by dissolving in 0.4 mL of distilled water for gastrogavage. The serum contents of hs-CRP were detected, with blood sample drawing from inferior vena cava, using enzyme-linked immunosorbent assay 17 weeks after medication. RESULTS: The hs-CRP level was significantly higher in Group B1 than in Groups A and C (P <0.05, P <0.01). Comparisons between various sub-groups of Group B in hs-CRP content showed that hs-CRP in Group B2, B4 and B7 was lower than that in B1 (P <0.01), hs-CRP in Group B4 was the lowest, and hs-CRP was lower in Group B2 than in Group B3. CONCLUSION: Assorted use of Chinese drugs for detoxifying and activating blood circulation could reduce serum hs-CRP level in ApoE (-/-) mice.