特应性皮炎患者血清CXC、CC及C亚家族趋化因子的检测

目的 了解部分CXC、CC及C亚家族趋化因子在特应性皮炎患者血清中的水平和相互关系，及与Th1及Th2相关的细胞因子的关联。方法 用双抗体夹心ELISA方法检测51例特应性皮炎患者血清γ-干扰素诱导的单核因子（Mig）、胸腺和活化调控的趋化因子（TARC）、皮肤T细胞趋化因子（CTACK）及淋巴细胞趋化因子（Ltn）等水平。以SCORAD对特应性皮炎患者进行评分。结果 特应性皮炎患者血清Mig、TARC、CTACK及Ltn水平均显著高于正常人对照组（P < 0.05或 < 0.01）。患者血清CTACK水平与SCORAD呈显著正相关；血清Mig、TARC、CTACK及Ltn水平均与皮损体表面积百分比（BSA%）呈正相关；患者血清Ltn与TARC和CTACK显著相关。结论 CXC、CC及C亚家族的趋化因子在特应性皮炎的发病中可能起作用。血清CTACK水平可能是评估特应性皮炎严重度的一个较好的指标。

Detection of serum CXC, CC and C chemokines in patients with atopic dermatitis

Objective To investigate the serum levels of CXC, CC and C chemokines, and to esti-mate the relationship among the three kinds of chemokines as well as that between these chemokines and other Th1 and Th2-related cytokines, in patients with atopic dermatitis (AD). Methods Fifty-one patients with atopic dermatitis, including 35 males and 16 females with an average age of 17 years and disease course of 13.6 years were enrolled into this study together with 34 normal human controls. ELISA was used to measure the serum levels of monokine induced by IFN-γ (Mig), thymus and activation-regulated chemokine (TARC), cutaneous T cell-attracting chemokine (CTACK) and lymphotactin (Ltn) in these subjects. Severity of AD was assessed according to SCORAD. Results The serum levels were 79.6±28.0 ng/L for Mig, 349.9±91.5 ng/L for TARC, 747.4±359.4 ng/L for CTACK and 141.0±68.4 ng/L for Ltn in patients, significantly higher than those in the normal controls (63.8±26.5 ng/L, 219.4±82.1 ng/L, 294.3± 64.9 ng/L, 80.9±54.2 ng/L, P < 0.05, 0.01, 0.01, 0.01 respectively). A significant correlation was observed between the serum level of CTACK and SCORAD score in patients (r = 0.343, P < 0.05). Similarly, the percentage of body surface area (BSA) involved positively correlated with the serum levels of Mig, TARC, CTACK and Ltn. Furthermore, there was a significant correlation between the serum level of Ltn and TARC (r=0.444, P< 0.01) as well as CTACK (r=0.572, P< 0.01), between that of CTACK and TARC (r=0.524, P< 0.01), and between that of TARC and Mig (r=0.313, P< 0.05). Conclusion The CXC, CC and C chemokines might be involved in the pathogenesis of AD. Further more, CTACK level could serve as a good indicator for the severity of AD.