双酚A及纳米二氧化钛对人胚肝L-02细胞内活性氧含量及DNA损伤的联合作用

目的]观察双酚A（BPA）、纳米二氧化钛（nano-TiO2）单独及联合作用对人胚肝L-02细胞内活性氧（reactive oxygen species，ROS）含量及DNA损伤的影响。方法]生长良好的L-02细胞，分别单独加入0．1、1、10μmol／L的BPA，1、5、10mg／L的nano-TiO2，以及上述各浓度的BPA和nano-TiO2混合染毒24h；采用流式细胞仪检测各组L-02细胞内ROS含量，采用彗星试验观察各组DNA断裂损伤程度。结果]10μmol／L BPA单独染毒组，1mg／L nano-TiO2与1、10μmol／L BPA，以及5、10mg／L的nano-TiO2分别与0．1、1、10μmol／L BPA联合作用于L-02细胞后，均可引起ROS含量升高，DNA断裂损伤加重，与阴性对照组比较差异有统计学意义（P〈0．001）；而nano-TiO2单独染毒组对于L-02细胞ROS的升高、DNA损伤作用与阴性对照组比较差异均无统计学意义（P〉0．05）。析因分析结果显示两种受试物混合染毒存在协同作用。结论]BPA和nano-TiO2联合作用可增加各自对L-02细胞内ROS水平和DNA损伤断裂水平的影响，对细胞的损伤作用增大。

Combined Effects of Bisphenol A and Nano-TiO2 on ROS and DNA Damage Level in Human Embryo Liver L-02 Cells

Objective ] To investigate the effects of Bisphenol A （ BPA ）, nano-TiO2, and BPA＋nano-TiO2 on ROS and DNA damage level in haman embryo liver L-02 cells. Methods ] L-02 cells were exposed to single BPA（0.1, 1, 10 μmol/L）, single nano-TiO2 （ 1, 5, 10 mg/L ）, and combination of BPA in different concentrations with nano-TiO2 respectively. Reactive oxygen species （ ROS ） level was determined by flow eytometry, and DNA damage was measured by comet assay. Results ] Compared with negative control group, increased ROS and DNA damage level were found in groups of 10 oanol/LBPA; 1 mg/L nano-TiO2 ＋ 1, 10 μmol/L BPA ; 5, 10 mg/L nano-TiO2 ＋ 0.1, 1, 10 μmol/L BPA, respectively.＇ No significant effects on ROS and DNA damage were observed in single nano-TiO2 groups （P 〉 0.05 ）. Coefficient effects on ROS and DNA damage were observed in the combined groups. Conclusion ] Combined usage of BPA and nano-TiO2 enhanced their capability in ROS induction and DNA damage effects on L-02 cells.