实时荧光PCR检测乙肝病毒DNA的效果评价

目的 观察应用实时荧光PCR检验法检测乙肝病毒DNA的效果，为临床提高诊断乙型肝炎病毒的准确性提供思路。方法 选取2015年11月~2018年7月于本院就诊的458例乙型肝炎患者作为研究对象，所有患者均经标准的abbot药盒检测确诊，其中大三阳患者241例，小三阳患者217例。患者入院后均采集空腹静脉血，制样本后采用酶联免疫吸附检验法和实时荧光PCR检验，比较两种检验方法对患者乙肝类型的阳性检出率。结果 实时荧光PCR检验法对大三阳的阳性检出率为95.43%，小三阳的阳性检出率为69.58%，均高于酶联免疫吸附检验法的85.06%、55.76%，差异有统计学意义（P＜0.05）。结论 实时荧光PCR检验法可直观的反映乙型肝炎病情状况，具有较高的诊断效果，临床医师可在实际工作中根据患者经济、病情等综合因素进行选用。

Evaluation of the Effect of Real-time Fluorescent PCR on Detection of Hepatitis B Virus DNA

Objective To observe the effect of real-time fluorescent PCR assay for detecting hepatitis B virus DNA, and to provide ideas for clinical improvement of hepatitis B virus accuracy. Methods A total of 458 patients with hepatitis B who were admitted to our hospital from November 2015 to July 2018 were enrolled in the study. All patients were diagnosed by standard abbot kit. Among them, 241 patients with Dasanyang patients and 217 patients with Xiaosanyang patients. example. Fasting venous blood was collected from all patients after admission. After the samples were prepared, enzyme-linked immunosorbent assay (ELISA) and real-time fluorescent PCR were used to compare the positive detection rates of the two test methods for patients with hepatitis B type. Results The positive detection rate of Dasanyang by real-time fluorescent PCR assay was 95.43%, and the positive detection rate of Xiaosanyang was 69.58%. Both were higher than 85.06% and 55.76% of the enzyme-linked immunosorbent assay (ELISA),the difference was statistically significant (P<0.05). Conclusion Real-time fluorescent PCR assay can directly reflect the status of hepatitis B, and has a high diagnostic effect. Clinicians can choose according to the comprehensive factors such as patient's economy and condition in actual work.