MTHFR C677T多态性与脓毒血症的关系

目的 探讨MTHFR C677T基因多态性（SNP）与脓毒血症的关系。方法 选择2014年1月~2018年6月我院住院的脓毒血症患者98例，采用梯度PCR及DNA测序技术，检测MTHFR C677T基因型，比较不同T淋巴细胞凋亡比例脓毒血症患者MTHFR C677T基因型分布情况及MTHFR C677T多态性与脓毒血症的关系。结果 MTHFR C677各位点基因型在T淋巴细胞中的凋亡比例方面比较，差异均无统计学意义（P＞0.05）；APACHEⅡ＜4分，SOFA＜2分的CT比例高于CC与TT基因型，降钙素原减少比例TT低于CC基因型与CT基因型，差异均有统计学意义（P＜0.05），28 天死亡人数比例TT低于CC基因型与CT基因型，差异均有统计学意义（P＜0.05）。结论 MTHFR C677T与脓毒血症患者预后有关，可根据MTHFR C677T的基因多态性预测脓毒血症的预后。

Relationship between MTHFR C677T Polymorphism and Sepsis

Objective To investigate the relationship between MTHFR C677T gene polymorphism (SNP) and sepsis.Methods 98 patients with sepsis hospitalized in our hospital from January 2014 to June 2018 were selected. Gradient PCR and DNA sequencing techniques were used to detect the MTHFR C677T genotype and compare the proportion of different T lymphocyte apoptosis patients with sepsis. The distribution of MTHFR C677T genotype and the relationship between MTHFR C677T polymorphism and sepsis.Results There was no significant difference in the proportion of MTHFR C677 genotypes in T lymphocytes (P>0.05). APACHEII<4 points, SOFA<2 points had higher CT ratio than CC and TT genotypes. The proportion of procalcitonin reduction was lower than CC genotype and CT genotype,the difference was statistically significant (P<0.05). The proportion of deaths in 28 days was lower than that of CC genotype and CT genotype,the difference was statistically significant (P<0.05). Conclusion MTHFR C677T is associated with the prognosis of patients with sepsis. The prognosis of sepsis can be predicted based on the gene polymorphism of MTHFR C677T.