IL-23在慢性乙型肝炎免疫调节中的作用研究

目的 探讨IL-23在慢性乙型肝炎免疫调节中的作用。方法 选取我院收治的32例HBeAg阳性慢性乙型肝炎患者为研究对象，根据ALT水平分为ALT≥120 IU/ml患者16例，ALT＜120 IU/ml患者16例，另外选择我院健康体检中心20例体检者作为健康对照组，采用酶联免疫分析法（ELISA）检测血清IL-23水平，采用流式细胞仪检测Th17细胞百分率。结果 与健康对照组相比，HBeAg阳性慢性乙型肝炎患者血清IL-23表达及外周血Th17细胞百分率均升高，差异有统计学意义（P＜0.05）； ALT≥120 IU/ml患者血清IL-23浓度（394.81±101.84）pg/ml高于ALT＜120IU/ml的（283.69±85.65）pg/ml，ALT≥120 IU/ml患者Th17细胞百分率（3.25±0.70）%高于ALT＜120IU/ml的（2.68±0.61）%，差异均有统计学意义（P＜0.05）；慢性乙型肝炎患者外周血Th17细胞百分率、血清IL-23浓度与ALT程度呈正相关（P均＜0.05）；Th17细胞百分率与血清IL-23浓度呈正相关（P＜0.05）。结论 IL-23可能通过影响Th17细胞的免疫调节参与慢性乙型肝炎患者炎症，为临床提供了新的靶标。

The Role of IL-23 in the Immune Regulation of Chronic Hepatitis B

Objective To investigate the role of IL-23 in the immunomodulation of chronic hepatitis B.Methods 32 patients with HBeAg-positive chronic hepatitis B admitted to our hospital were enrolled. According to the ALT level, 16 patients with ALT≥120 IU/ml and 16 patients with ALT<120 IU/ml were selected. 20 patients in the center were used as healthy controls. Serum IL-23 levels were measured by enzyme-linked immunosorbent assay (ELISA), and the percentage of Th17 cells was detected by flow cytometry.Results Compared with the healthy control group, the expression of serum IL-23 and the percentage of peripheral blood Th17 cells in HBeAg-positive chronic hepatitis B patients were significantly increased (P<0.05). Serum IL in patients with ALT≥120 IU/ml -23 concentration (394.81±101.84) pg/ml was higher than ALT<120 IU/ml (283.69±85.65) pg/ml, ALT≥120IU/ml patients with Th17 cell percentage (3.25±0.70)% higher than ALT<120 IU/ml (2.68±0.61)%, the difference was statistically significant (P<0.05); the percentage of Th17 cells and serum IL-23 in peripheral blood of patients with chronic hepatitis B were positively correlated with the degree of ALT (P<0.05); Th17 cells The percentage was positively correlated with serum IL-23 concentration (P<0.05). Conclusion IL-23 may participate in the inflammation of patients with chronic hepatitis B by affecting the immune regulation of Th17 cells, providing a new target for clinical.