PNB/TCH生长试验和DNA微阵列基因芯片鉴定非结核分枝杆菌的价值比较

目的 比较对硝基苯甲酸/噻吩-2-羧酸肼生长（PNB/TCH）和DNA微阵列基因芯片鉴定非结核分枝杆菌（NTM）临床分离株菌种的价值。方法 收集2013年1月~2018年6月我院住院患者的痰分枝杆菌培阳分离菌2175株，分别采用PNB/TCH生长试验和DNA微阵列基因芯片鉴别NTM，比较两种方法的鉴定结果。结果 PNB/TCH生长试验初步鉴定NTM 93株，占4.28%，DNA微阵列基因芯片鉴定NTM 79株，占3.63%，PNB/TCH生长试验初步鉴定的93株NTM中，有14例为假NTM（15.05%）；PNB/TCH生长试验鉴定NTM的阳性检出率低于DNA微阵列基因芯片；79株NTM中，占比前3位的依次为胞内分枝杆菌株（37.97%）、堪萨斯分枝杆菌（21.52%）、鸟分枝杆菌（16.46%）。结论 传统对硝基苯甲酸/噻吩-2-羧酸肼生长试验鉴定NTM特异性较低，采用DNA微阵列基因芯片技术鉴定NTM准确，临床参考价值较大。

Comparison of PNB/TCH Growth and DNA Microarray Gene Chips for Identification of Nontuberculous Mycobacteria

Objective To compare the growth of non-tuberculous mycobacteria (NTM) clinical isolates with p-nitrobenzoic acid/thiophene-2-carboxylate growth (PNB/TCH) and DNA microarray microarray.Methods A total of 2175 strains of Mycobacterium phlei isolates in our hospital from January 2013 to June 2018 were collected. PNB/TCH growth test and DNA microarray gene chip were used to identify NTM. Identification of the method.Results PNB/TCH growth assay initially identified NTM 93 strain, accounting for 4.28%, DNA microarray gene chip identified NTM 79 strain, accounting for 3.63%, and of the 93 NTMs initially identified by PNB/TCH growth test, 14 were pseudo NTM ( 15.05%); PNB/TCH growth test identified that the positive detection rate of NTM was lower than that of DNA microarray gene chip; among the 79 strains of NTM, the top 3 were followed by intracellular branching strain (37.97%), Kansas Mycobacterium (21.52%), Mycobacterium avium (16.46%). Conclusion The traditional p-nitrobenzoic acid/thiophene-2-carboxylic acid oxime growth test has low specificity of NTM. The DNA microarray gene chip technology is used to identify NTM accurately, and the clinical reference value is large.