MiR-145-5p对胶原诱导的关节炎小鼠关节炎症的影响

目的:探讨miR-145-5p对胶原诱导的关节炎（CIA）小鼠关节变化的影响。方法:采用牛Ⅱ型胶原免疫DBA/1小鼠诱导CIA，对关节指数评分及足跖肿胀程度进行比较，成模后随机分为agomiR-145-5p组和agomiR空载体（agomiR-NC）组进行干扰，采用足爪micro-CT摄片，踝关节病理检查评分及石蜡切片、HE染色进行处理，并通过免疫组化方法（IHC）检测踝关节中金属蛋白酶-1（MMP-1）、金属蛋白酶-3（MMP-3）、金属蛋白酶-9（MMP-9）、金属蛋白酶-13（MMP-13）、白细胞介素17（IL-17）和金属蛋白酶17（ADAM17）的表达。结果:成功建立CIA小鼠模型，与agomiR-NC组相比，agomiR-145-5p组micro-CT分析显示骨侵蚀更严重，组织病理学显示滑膜炎症减轻，免疫组化结果显示MMP-3、MMP-9、MMP-13和IL-17水平升高， MMP-1水平无差异，ADAM17水平降低（P<0.05）。结论:成功建立CIA小鼠模型，并发现miR-145-5p加重关节破坏的同时部分抑制了CIA小鼠的关节炎症。

Effects of miR-145-5p on joint inflammation in collagen-induced arthritis mice

Objective: To investigate the effect of miR-145-5p on joint changes in collagen-induced arthritis (CIA) mice. Methods:The CIA was induced by immunization of bovine type II collagen in DBA/1 mice, and the joint index scores and degree of paw swelling were compared. After the model was formed, they were randomly divided into agomiR-145-5p group and agomiR-NC group toperform interference.Foot micro-CT radiographs, ankle pathology score, paraffin sections, and HE staining were measured. IHC was used to detect the expression of MMP-1, MMP-3, MMP-9, MMP-13, IL-17 and ADAM17 in the ankle joint. Results:The CIA mouse model was successfully established. Compared with the agomiR-NC group, bone erosion was more severe in the agomiR-145-5p group. Histopathology showed synovial inflammation was reduced, and immunohistochemistry showed MMP-3 and MMP-9, MMP-13 and IL-17 levels increased, MMP-1 levels were not different, and ADAM17 levels decreased (P<0.05). Conclusion: We have successfully established a CIA mouse model and found that miR-145-5p could aggravate joint destruction while partially inhibiting joint inflammation in CIA mice.