MiR-199a-3p在胰腺癌中的表达水平及生物信息学分析

目的:探讨miR-199a-3p在胰腺癌中的表达水平及作为胰腺癌的诊断指标的意义，并进行生物信息学分析。方法:从GEO数据库收集胰腺癌和正常胰腺组织中miR-199a-3p的数据。汇总数据绘制受试者工作特征曲线，进行Meta分析，采用生物信息学方法预测miR-199a-3p的靶基因，并对靶基因进行GO功能富集分析和KEGG通路富集分析。结果:收集到6个GEO数据，4个数据的ROC曲线表现出显著的诊断意义，Meta分析结果显示miR-199a-3p在胰腺癌中表达水平高于正常胰腺组织，差异有统计学意义（P<0.001）。预测到221个miR-199a-3p潜在的靶基因，其功能主要富集在基因表达的调控，细胞代谢的调控，蛋白质域特异性结合等生物学过程（P<0.001），KEGG分析结果显示主要集中在PI3K-Akt通路、黏着斑、肌动蛋白细胞骨架调控、癌症通路、Hippo通路、鞘脂通路、ErbB通路、MAPK通路等通路（P<0.05）。结论:相比正常胰腺组织，miR-199a-3p在胰腺癌为高表达，可作为胰腺癌的一个潜在诊断指标，并可能在胰腺癌的发生发展中起到重要作用，为胰腺癌的研究和治疗提供了新的方向。

The expression of miR-199a-3p in pancreatic cancer and bioinformatics analysis

Objective: To investigate miR-199a-3p’s expression levels and diagnostic value in pancreatic cancer, and to conduct bioinformatics analysis. Methods:We gathered miR-199a-3p expression data of pancreatic cancer and normal pancreatic tissues by analysis of a public dataset from Gene Expression Omnibus (GEO) databases. We plotted ROC curve and conducted Meta-analysis using all collected data. Furthermore, the bioinformatics analysis of miR-199a-3p involved target prediction analysis, gene ontology enrichment and KEGG pathway enrichment. Results: A total of six GEO data were finally collected in this study, four of these data showed significant diagnostic value by conducting ROC curve，and Meta-analysis indicated that miR-199a-3p expression was significantly higher in pancreatic cancer by GEO data compared with normal pancreatic tissue (P<0.001). The 221 target genes were predicted as potential target genes for miR-199a-3p. The function of target genes was mostly enriched in the regulation of cell metabolism, regulation of gene expression, protein domain specific binding and other biological processes(P<0.001). KEGG analysis was mostly observed in enrichment pathway, such as PI3K-Akt signaling pathway, focal adhesion, actin cytoskeleton regulation, cancer pathway, Hippo signaling pathway, sphingolipid signaling pathways, ErbB signaling pathway, MAPK signaling pathway and other Signal pathways (P<0.05). Conclusion: MiR-199a-3p expression is significantly higher in pancreatic cancer compared with normal pancreatic tissues. It can be used as a potential diagnostic biomarker of pancreatic cancer, and it may play a vital role in the development of pancreatic cancer. Meanwhile, it could provide a research direction for the treatment of pancreatic cancer in the future.