埃兹蛋白在乳腺癌中的表达及其临床意义

目的 通过检测埃兹蛋白在浸润性乳腺癌、乳腺导管内癌及正常乳腺组织中的表达，探究埃兹蛋白在乳腺癌组织中的表达的临床意义。方法 随机选取南昌大学第一附属医院40例浸润性乳腺癌患者及32例乳腺导管内癌患者，另外选择同期来我院健康体检者22例。通过免疫组化链霉亲和素-过氧化物酶法（S-P）法分别检测埃兹蛋白在22例正常乳腺组织、32例乳腺导管内癌组织和40例浸润性乳腺癌组织中的表达，参照病理结果进行分析。结果 埃兹蛋白在40例浸润性乳腺癌的阳性表达率为72.50%、在32例乳腺导管内癌的阳性表达率为40.63%、在正常乳腺组织中的阳性表达率13.64%，浸润性乳腺癌中埃兹蛋白的表达高于其他两种组织，差异具有统计学意义（P＜0.05）；埃兹蛋白在G3期乳腺癌组织中的表达高于在G1~G2期乳腺癌组织中的表达，差异具有统计学意义（P＜0.05）；埃兹蛋白在淋巴结转移患者中的阳性表达率高于在无淋巴结转移的患者中的阳性表达率，差异统计学意义显著（P＜0.01）；埃兹蛋白在＜40岁的患者中的阳性表达率低于在年龄≥40岁的患者中的表达率，差异统计学意义显著（P＜0.01）；埃兹蛋白在PR阳性患者中的阳性表达率低于在PR阴性患者中的表达率，差异具有统计学意义（P＜0.05）；乳腺癌患者中埃兹蛋白的表达与组织学分级、腋窝淋巴结转移及年龄呈正相关（P＜0.05），与PR呈负相关（P＜0.05）；埃兹蛋白在浸润性润腺癌中的表达与乳腺癌临床分期、脉管侵犯、肿瘤大小、患者月经状态以及Her-2、ER、Ki-67的表达无相关性（P＞0.05）。结论 埃兹蛋白在乳腺癌和正常乳腺组织中的表达不同，对判断乳腺肿瘤性质具有重要参考意义；此外，埃兹蛋白能够帮助判断乳腺癌的转移潜能和预后，可能启发乳腺癌新的靶向治疗。

Expression of Ezrin in Breast Cancer and Its Clinical Significance

Objective To investigate the clinical significance of ezrin expression in breast cancer tissues by detecting the expression of ezrin in invasive breast cancer, intraductal carcinoma of the breast and normal breast tissue. Methods 40 patients with invasive breast cancer and 32 patients with intraductal carcinoma of the breast in the First Affiliated Hospital of Nanchang University were randomly selected. In addition, 22 healthy subjects were enrolled in our hospital. The expression of ezrin in 22 normal breast tissues, 32 breast ductal carcinoma tissues and 40 invasive breast cancer tissues was detected by immunohistochemical streptavidin-peroxidase (S-P) method. The analysis was performed with reference to the pathological results. Results The positive expression rate of ezrin in 40 cases of invasive breast cancer was 72.50%, the positive expression rate in 32 cases of breast ductal carcinoma was 40.63%, and the positive expression rate in normal breast tissue was 13.64%.The expression of ezrin in invasive breast cancer was higher than that in the other two tissues,the difference was statistically significant (P<0.05); the expression of ezrin in G3 breast cancer tissues was higher than that in G1~G2 breast cancer tissues, the difference was statistically significant (P<0.05); The positive expression rate of ezrin in patients with lymph node metastasis was higher than that in patients without lymph node metastasis, the difference was statistically significant (P<0.01); The positive expression rate of ezrin in patients <40 years old was lower than that in patients aged 40 years or older, the difference was statistically significant (P<0.01); the positive expression of ezrin in PR positive patients the rate was lower than that in PR-negative patients,the difference was statistically significant (P<0.05). The expression of ezrin in breast cancer patients was positively correlated with histological grade, axillary lymph node metastasis and age (P<0.05),negative correlation with PR (P<0.05); expression of ezrin in invasive adenocarcinoma and clinical stage of breast cancer, vascular invasion, tumor size, patient's menstrual status and Her-2, ER, Ki-67 there was no correlation in expression (P>0.05). Conclusion The expression of ezrin in breast cancer and normal breast tissue is different, which has important reference significance for judging the nature of breast tumor. In addition, ezrin can help determine the metastatic potential and prognosis of breast cancer, and may inspire new targets of breast cancer treatment.