惊恐障碍患者血清VGF水平的对照研究

目的 探讨惊恐障碍患者血清VGF水平的变化，分析其与疾病临床症状的关系及其对惊恐障碍的辅助诊断价值。方法 选择东南大学附属中大医院心理精神科2015年4月~2016年8月住院的惊恐障碍患者30例作为研究对象，设为惊恐障碍组。另选同期东南大学附属中大医院体检中心的健康体检者30例设为对照组。采用酶联免疫吸附实验（ELISA）检测血清VGF蛋白水平，同时采用惊恐障碍严重程度量表（PDSS）、汉密尔顿焦虑量表（HARS）对所有研究对象进行评估；采用Spearman相关分析对血清VGF水平与焦虑评分、惊恐症状严重程度进行相关性分析，并使用受试者工作特征（ROC）曲线评价VGF辅助诊断惊恐障碍的价值。结果 惊恐障碍组血清VGF水平为（874.16±42.84）pg/ml，低于对照组的（1041.47±53.74）pg/ml，差异有统计学意义（P＜0.05）。相关分析未发现血清VGF水平与惊恐障碍的严重程度、HARS得分等临床变量之间存在相关性（P＞0.05）。ROC分析显示，血清VGF辅助诊断惊恐障碍的阈值（cut-off）为972.19 pg/ml，对应的ROC曲线下面积（AUC）为0.999，特异性为96.70%，敏感性达100.00%。结论 血清VGF水平在惊恐障碍患者中显著降低，具有辅助诊断惊恐障碍的价值。

Controlled Study of Serum VGF Levels in Patients with Panic Disorder

Objective To investigate the changes of serum VGF levels in patients with panic disorder, and to analyze its relationship with clinical symptoms of the disease and its auxiliary diagnostic value for panic disorder. Methods 30 patients with panic disorder who were hospitalized from April 2015 to August 2016 in the Department of Psychology and Psychiatry of Zhongda Hospital affiliated to Southeast University were selected as the research objects and set as panic disorder group. In the same period, 30 healthy medical examiners from the Medical Center of Zhongda Hospital of Southeast University were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum VGF protein levels, while panic disorder severity scale (PDSS) and Hamilton anxiety scale (HARS) were used to evaluate all subjects. Spearman correlation analysis was used to determine serum VGF levels and Correlation analysis was performed on anxiety score and panic symptoms severity, and the receiver operating characteristic (ROC) curve was used to evaluate the value of VGF in diagnosing panic disorders.Results The level of serum VGF in panic disorder group was (874.16 ± 42.84) pg/ml, which was lower than that in the control group (1041.47 ± 53.74) pg/ml, the difference was statistically significant (P<0.05). Correlation analysis found no correlation between serum VGF levels and clinical variables such as the severity of panic disorder and HARS score (P> 0.05).ROC analysis showed that the(cut-off) threshold of serum VGF for diagnosis of panic disorder was 972.19 pg/ml, the area under the corresponding ROC curve (AUC) was 0.999, the specificity was 96.70%, and the sensitivity was 100.00%. Conclusion Serum VGF level is significantly reduced in patients with panic disorder, which has the value of assisting the diagnosis of panic disorder.