肠通对小鼠便秘的有效性研究

目的 研究肠通对小鼠便秘的有效性。方法 用复方地芬诺酯灌胃制备小鼠便秘模型100只，用0.9% NS灌胃制备空白组模型20只，分为肠通高剂量组（10 mg/只）、肠通中剂量组（5 mg/只）、肠通低剂量组（2.5 mg/只）、阳性对照组、模型组和空白组共6组，每组20只。肠通高剂量组、中剂量组、低剂量组用肠通与墨汁等体积混合进行灌胃，0.1 ml/只；阳性对照组将杜密克与墨汁等体积混合灌胃，67 mg/只，0.1 ml/只；模型组和空白组用墨汁灌胃，0.1 ml/只。灌胃25 min后，每组脱颈处死10只测定墨汁推进率，取小肠标本做病理检查。每组存留的10只小鼠观察灌胃后首粒排黑便时间、6 h内排黑便粒数、粪便含水量、有无死亡。结果 ①肠通对便秘小鼠墨汁推进率的影响:各组小鼠小肠总长度比较，差异无统计学意义（P＞0.05）；空白组墨汁推进长度、墨汁推进率均大于其余各组，肠通高、中、低剂量组均大于模型组，差异有统计学意义（P＜0.05）。②肠通对便秘小鼠排便情况的影响:空白组首粒排便时间短于其余各组，差异有统计学意义（P＜0.05）；模型组6 h排便粒数、排便含水量均少于其余各组，差异有统计学意义（P＜0.05）。③各组小鼠均未死亡。④病理学:各组小肠绒毛排列整齐，结构完整。结论 肠通能有效治疗小鼠便秘。

Effect of Changtong on Constipation in Mice

Objective To study the effectiveness of Changtong on constipation in mice. Methods A total of 100 mice constipation models were prepared by intragastric administration of compound diphenoxylate. 20 mice in the blank group were prepared by intragastric administration of 0.9% NS. They were divided into high-dose group (10 mg/mouse),Intestinal medium dose group (5 mg/mouse), intestinal low dose group (2.5 mg/mouse), positive control group, the model group and the blank group, 20 mice in each group. The high-dose group, the middle-dose group and the low-dose group were intragastrically administered with an equal volume of intestinal and ink, 0.1 ml/mouse; the positive control group was mixed with Dumilk and ink in the same volume, 67 mg/mouse. 0.1 ml/mouse; model group and blank group were intragastrically administered with 0.1 ml/mouse. After 25 minutes of gavage, 10 mice in each group were sacrificed to measure the ink propellation rate, and the small intestine specimens were taken for pathological examination. 10 mice in each group were observed for the first squatting time after gavage, the number of black stools in 6 h, the moisture content of the feces, and the presence or absence of death. Results ①The effect of Changtong on the ink propelling rate of constipation mice: There was no significant difference in the total length of small intestine between the groups (P>0.05); the ink propulsion length and ink propulsion rate of the blank group were larger than the other groups, The high, medium and low dose groups were larger than the model group,the difference was statistically significant (P<0.05).②The effect of Changtong on the defecation of constipation mice: The first defecation time in the blank group was shorter than the other groups, the difference was statistically significant (P<0.05); The number of defecation and defecation water content in the model group were lower than those in the other groups at 6 h,the difference was statistically significant (P<0.05).③None of the mice in each group died. ④Pathology: The villi of the small intestines were arranged neatly and the structure was complete. Conclusion Changtong can effectively treat constipation in mice.