特应性皮炎患儿与正常儿童肠道菌群差异性研究

【摘要】 目的 探讨特应性皮炎（AD） 患儿与正常儿童肠道菌群的差异。方法 收集2015年4月至2017年4月在上海市嘉定区中医医院皮肤科门诊就诊的AD患儿35例，以27例健康儿童作为对照组。取受试者粪便，提取总DNA后，PCR扩增细菌的16SrRNA基因V3 ~ V4区，应用Illumina Miseq测序平台行高通量测序，分析菌群丰度差异。选择两组丰度排名前15的门、属、种分别比较物种差异，采用Wilcoxon秩和检验。结果 两组肠道菌群主要由厚壁菌门、拟杆菌门、变形菌门、放线菌门组成。在菌门水平，AD组与健康对照组拟杆菌门丰度分别为29.16% ± 19.96%、39.06% ± 15.98%（P = 0.042），梭杆菌门分别为0.06% ± 0.17%、0.50% ± 1.71%（P = 0.041）；在菌属水平，两组拟杆菌属丰度分别为23.77% ± 18.08%、33.1% ± 15.75%（P = 0.029）；在菌种水平，丰度排名前15的菌种两组间分布差异均无统计学意义（均P > 0.05）。结论 特应性皮炎患儿与正常儿童肠道菌群构成及菌群相对丰度存在一定差异。

Difference in intestinal microbiome between children with atopic dermatitis and healthy children

【Abstract】 Objective To investigate the difference in intestinal microbiome between children with atopic dermatitis （AD） and healthy children. Methods Totally, 35 children with AD were enrolled from the Department of Dermatology, Jiading Hospital of Traditional Chinese Medicine from April 2015 to April 2017, and 27 healthy children served as control group. Total DNA was extracted from the feces of the subjects, and the V3-V4 region of the 16S rRNA gene of the bacteria was amplified by PCR. High throughput sequencing was performed using the Illumina Miseq sequencing platform to analyze the diversity of bacterial flora. The top 15 abundant bacteria at phylum, genus, and species levels respectively were selected in and compared between the two groups. Statistical analysis was carried out using Wilcoxon rank sum test. Results The intestinal microbiome in the two groups mainly consisted of Firmicutes, Bacteroidetes, Proteobacteria and Actinobacteria. At the phylum level, the abundance of Bacteroidetes and Fusobacteria was significantly lower in the AD group （29.16% ± 19.96%, 0.06% ± 0.17%） than in the healthy control group （39.06% ± 15.98%, 0.50% ± 1.71%, respectively, P = 0.042, 0.041）. At the genus level, the abundance of Bacteroides was significantly lower in the AD group （23.77% ± 18.08%） than in the healthy control group （33.1% ± 15.75%, P = 0.029）. There was no significant difference in the distribution of the top 15 abundant species between the two groups （all P > 0.05）. Conclusion There are some differences in the composition of intestinal microbiome and relative abundance of bacteria between children with AD and healthy children.