| 赤芍中氧化芍药苷和芍药苷的含量测定 |
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| 引用本文： | 冀兰鑫,傅俊曾,黄浩,罗国安.赤芍中氧化芍药苷和芍药苷的含量测定[J].亚太传统医药,2010,6(7):18-19. |
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| 作者姓名： | 冀兰鑫 傅俊曾 黄浩 罗国安 |
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| 作者单位： | 1. 南开大学,药学院,天津,300071
2. 南开大学,药学院,天津,300071;清华大学,化学系,北京,100084 |
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| 摘 要： | 目的:建立同时测定赤芍中氧化芍药苷和芍药苷含量的HPLC方法。方法:采用反向高效液相色谱法,使用AglientZorbaxSB-C18(4.6mm×250mm,5μm)色谱柱,流动相为乙腈—水,流速1mL/min,检测波长254nm,柱温35℃,进样量20μL。结果:氧化芍药苷回归方程为Y=41607X-164942,R=0.9994,线性范围为0.02525～0.20200mg/mL;芍药苷回归方程为Y=3641.6X+174622,R=0.9995,线性范围为0.2525～2.0200mg/mL。结论:建立了一个测定赤芍中氧化芍药苷和芍药苷含量的方法。
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| 关 键 词： | 赤芍 氧化芍药苷 芍药苷 高效液相色谱 含量测定 |
Determination of oxypaeoniflorin and paeoniflorin in Radix Paeoniae Rubra |
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| Ji Lanxin,Fu Junzeng,Huang Hao,Luo Guo'an.Determination of oxypaeoniflorin and paeoniflorin in Radix Paeoniae Rubra[J].Asia-Pacific Traditional Medicine,2010,6(7):18-19. |
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| Authors: | Ji Lanxin Fu Junzeng Huang Hao Luo Guo'an |
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| Institution: | Ji Lanxin,Fu Junzeng, Huang Hao, Luo Guo＇an (1.College of Pharmacy,Nankai University,Tianjin 300071,China;2.Department of Chemistry,Tsinghua University, Beijing 100084,China.) |
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| Abstract: | Objective： To establish a high performance liquid chromatographic （HPLC） method for simultaneous determination of oxypaeoniflorin and paeoniflorin in Radix Paeoniae Rubra. Method： An Aglient Zorbax SB-C18 column （4.6 min×250 mm, 5 μm） was used. The mobile phase consisted of aeetonitfile - water; the flow rate was 1 mL/min; the detector wavelength set at 254 nm; the column temperature was 35 ℃; injection volume was 20 μL. Results： The standard curves for oxypaeoniflorin was linear in the range of 0.02525-0.20200mg/mL,the regression equation being Y=41607X-164942, R=0.9994; the standard curves for paeoniflorin was linear in the range of 0.2525-2.0200mg/mL, the regression equation being Y=3641.6X＋174622, R=0.9995. Conclusion： A method suitable for determination of oxypaeoniflorin and paeoniflorin in Radix Paeoniae Rubra was established. |
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| Keywords: | Radix Paeoniae Rubra Oxypaeoniflorin Paeoniflorin HPLC Content Determination |
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