High Cell-Density Culture System of Hepatocytes Entrapped in a Three-Dimensional Hollow Fiber Module with Collagen Gel |
| |
| Authors: | Kazuyoshi Takeshita Haruaki Ishibashi Masayuki Suzuki Takumi Yamamoto Toshihiro Akaike Masashi Kodama |
| |
| Affiliation: | First Department of Surgery, Shiga University of Medical Science, Otsu, Shiga, Japan;First Department of Tokyo Institute of Technology, Tokyo, Japan |
| |
| Abstract: | Abstract: A compact three-dimensional (3D) module is needed for hepatocyte culture in order to develop an effective hybrid artificial liver system that can retain hepa-tocellular structure and differentiated functions. We treated the 3D module with collagen gel to entrap rat hepatocytes. This method yielded a high hepatocellular density (2 times 107 cells/ml) over a period of 14 days and maintained the secretion of albumin and ureogenesis at the same level as the control monolayer method. The ammonia removal remained at 43% of the Day 0 value over 8 days of perfusion. Our data show that this approach may be useful for liver support therapy in an ex-tracorporeal circuit. |
| |
| Keywords: | Hepatocyte High cell density Perfusion culture Collagen gel Hybrid artificial liver |
|
