黄芪多糖对淋巴细胞与血管内皮细胞粘附的影响及其分子机制

目的 从影响淋巴细胞与血管内皮细胞粘附的角度探讨黄区欧糖免疫增强作用的机制。方法 以体外培养的人脐静脉内皮细胞（ＨＵＶＥＣ）为模型，应用蛋杂料染色、细胞ＥＬＩＳＡＳ、免疫细胞化学等方法。研究黄芪多糖对淋巴细胞与血管内皮细胞粘附的影响及其分子机制。结果 黄芪多糖作用于ＨＵＶＥＣ，能促进ＨＵＶＥＣ与淋巴细胞粘附，并与ＩＬ－１、ＴＮＦ有协同增强作用，其主要分子机制为促进ＨＵＶＥＣ表面粘附分子ＣＡＭ－１的

Effect of astragalus polysaccharin (APS)on lymphocyte-endothelium adhesion and the molecular mechanism

ObjectiveTo investigate the effect of APS on lymphocyteendothelium adhesion and cell adhesion molecules(CAMs)Methods Based on the model of human umbilical vein endothelial cell(HUVEC),this study adopted Rose Bengal Stain,cell ELISA,immunocytochemistry techniques to investigate the effect of APS on lymphocyteendothelium adhesion and the molecular mechanismResults Incubation of HUVEC with APS markedly enhanced HUVEC adherence for lymphocyte and APS had synergism with IL1 and TNFThe main molecular mechanism is that APS upregulate the expression of ICAM1 on the HUVECThe lymphocyte treated with APS did not show increase in their adherence to HUVEC,and had no upregulation of CD18 expressionConclusion It is one of the mechanisms of immunopotentiation of APS that it promote lymphocyteendothelium adhesion by upregulating the CAMs expression to promote lymphocyte recirculation