基质金属蛋白酶-9和血管内皮生长因子与原发性肝癌侵袭转移关系研究

目的　探讨原发性肝癌中MMP 9和VEGF基因的表达 ,探讨其与原发性肝癌侵袭转移的关系。方法　采用逆转录 聚合酶链式反应 (RT PCR)技术 ,检测 41例原发性肝癌手术切除肿瘤组织、癌旁组织标本的MMP 9和VEGF基因表达。结果　肝癌组织和癌旁 1cm、5cm组织中分别有 70 7%、48 9%、41 5 %MMP 9表达阳性和 75 6%、5 3 7%、46 3 %VEGF表达阳性 ,癌组织的MMP 9和VEGF表达水平显著高于癌旁 5cm组织 (P <0 0 1) ,癌组织与癌旁 1cm组织MMP 9和VEGF表达水平无显著性差异。癌组织中MMP 9和VEGF的表达水平与有无包膜、门静脉癌栓有显著性差异 (P <0 0 5 )。MMP 9与复发、AFP阳性有显著性差异 (P <0 0 5 ) ,而VEGF与之无差异。结论　MMP 9和VEGF基因的过度表达与肝癌侵袭转移密切相关 ,MMP 9可作为判断预后的指标

Significance of MMP and VEGF mRNA expression in invasion and metastasis of hepatocellular carcinoma

Objective To investigate the expression of MMP 9 and VEGF genes in primary hepatocellular carcinoma (HCC) and explore its relation to invasion and metastasis of the tumor. Methods RT PCR was used to detect MMP 9 and VEGF mRNA in surgically extracted specimens from 41 patients with HCC. Results The rate of positive expression of MMP 9 was 70 7%, 48 9% and 41 5% while that of VEGF was 75 6%, 53 7% and 46 3% in tumor tissue, 1 cm adjacent tissue and 5 cm adjacent tissue, respectively. The expression of MMP 9 and VEGF was significantly higher in tumor tissue than in 5 cm adjacent tissue (P<0 01). Meanwhile, the expression of MMP 9 and VEGF was significantly higher in tumors with pv emboli or poor encapsulated tumors than in those without pv emboli or well encapsulated tumors (P<0 05). The expression of MMP 9 was markedly related to HCC recurrence and AFP positivity. Conclusions The expression of MMP 9 and VEGF is closely correlated to recurrence or metastasis of HCC. MMP 9 expression level can be used as a predictor for prognosis of HCC.