| HPLC法测定刺五加注射液中紫丁香苷和异嗪皮啶的含量 |
| |
| 引用本文： | 隆颖,栗建明,莫结丽,廖弦.HPLC法测定刺五加注射液中紫丁香苷和异嗪皮啶的含量[J].中国药品标准,2011,12(6):410-412. |
| |
| 作者姓名： | 隆颖 栗建明 莫结丽 廖弦 |
| |
| 作者单位： | 1. 广东省药品检验所,广州,510180
2. 广州市药品检验所,广州,510160 |
| |
| 摘 要： | 目的:建立刺五加注射液中紫丁香苷和异嗪皮啶的含量测定方法.方法:采用HPLC法.色谱柱为Diamomsil C18 (4.6mm× 200 mm,5μm),甲醇-水(20∶80)为流动相,流速1.0 mL·min-1,检测波长(双波长)265 nm,344nm.结果:紫丁香苷在0.01308～1.308 μg,异嗪皮啶在0.014659～0.122 16 μg范围内呈良好的线性关系,平均回收率分别为98.7％和102.2％,RSD分别为1.0％和0.8％.结论:所用方法简便、准确,能有效控制该制剂的质量.
|
| 关 键 词： | 刺五加注射液 紫丁香苷 异嗪皮啶 |
Determination of Syringin and Isofraxidin in Ciwujia Injection by RP-HPLC |
| |
| Authors: | Long Ying Li Jian-ming Mo Jie-li and Liao Xian |
| |
| Institution: | 1. Guangdong Institute for Drug Control, Guangzhou 510180 ;2. Guangzhou Institute for Drug Control, Guangzhou 510160 ) |
| |
| Abstract: | Objective： To establish a method for the content determination of Syringin and Isofraxidin in Ciwujia Injection. Methods： The analysis was performed on a Diamomsil Cls column （4. 6 mm ×200 mm,5 μm）. The mobile phase consisted of methanol-water （20： 80）. The flow rate was 1.0 mL·min^-1. The detection wavelength was set at 265 nm and 344 nm. Results： The calibration curves showed good linear response ranged from 0. 013 08 - 1. 308 μg for Syringin and O. 0146 59 -0. 122 16μg for Isofraxidin. The average recoveries for yringin and Isofraxidin were 98. 7% （RSD = 1.0% ） and 102. 2% （ RSD =0. 8% ）, respectively. Conclusion： This method is simple and reliable for quality control of Ciwujia Injection. |
| |
| Keywords: | Ciwujia Isnjection Syringin Isofraxidin |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药品标准》浏览原始摘要信息 |
| 点击此处可从《中国药品标准》下载免费的PDF全文 |
|
