奥曲肽诱导肝癌细胞凋亡和Fas/FasL的表达

目的 探讨奥曲肽对人肝癌细胞抑制作用的机制,为奥曲肽临床应用提供实验依据.方法 用奥曲肽4、7、10 μ g/ml作用人肝癌细胞株(SMMC-7721)24、48、72 h,以空白作为对照组;采用原位末端标记(TUNEL)法流式细胞仪检测SMMC-7721凋亡;直接标记单克隆(CD95/CDl78)抗体法流式细胞仪检测SMMC-7721 Fas/FasL表达的变化.结果 奥曲肽在4、7、10 μg/ml d作用 SMMC-7721 24 h后,SMMC-7721凋亡率从8%升高为40%(P＜0.05),Fas表达率从57%升高为76%(P＜0.05),FasL表达率从54%升高为63%(P＜0.05).结论 奥曲肽能诱导肝癌细胞(Hep SMMC-7721)凋亡的发生,而且能促进肝癌细胞Fas/Fasl基因的表达.

Octreotide induces apoptosis of human hepatoma cells by the mechanism of facilitating the Fas/FasL gene expression therein

OBJECTIVE: To investigate the effects of octreotide on apoptosis of and Fas/FasL gene expression in human hepatoma cell mechanism of the suppression effect of liver cancer with e in order to offer the experimental foundation for clinic treatment. METHODS: Hyman hematoma cells of the line SMMC-7721 were cultured and divided into two groups: octreotide group, co-cultured with octreotide 4,7, and 10 microg/ml respectively for 24 h, 48 h, or 72 h, and control group. TUNEL was used to detect the apoptosis of the cells. Flow cytometry was used with terminal deoxynucleatidyl transferase mediated dUTP nick end labeling (TUNEL) technique to detect the apoptosis of the cells. FC was used with direct labeling of monoclonal antibody (CD95/CD178) to detect the expression of Fas and FasL, and the Fas/FasL ratio. RESULTS: Octreotide increased the apoptotic rate of the cells dose- and time-dependently (all P < 0.05). Octreotide increased the Fas expression rate, FasL expression rate, and Fas/FasL ratio dose- and time-dependently (all P < 0.05). CONCLUSION: Octreotide induces the apoptosis of human hepatoma cells, possibly by the mechanism of facilitating the Fas/FasL gene expression.