阿奇霉素对哮喘豚鼠气道炎症核因子-Kappa B的表达研究

目的研究不同剂量阿奇霉素（Azithromycin,AZM）对哮喘豚鼠肺组织核因子-Kappa B（NF-κB）的表达影响。方法将40只健康雄性豚鼠随机分成对照组（A组,n=8）、哮喘组（B组,n=8）、阿奇霉素低剂量治疗组（C组,n=8）、阿奇霉素中剂量治疗组（D组,n=8）、阿奇霉素高剂量治疗组（E组,n=8）。经鸡卵白蛋白（OVA）致敏后,给予OVA雾化吸入,制作哮喘动物模型。C组给予阿奇霉素25 mg/（kg.d）,D组及E组分别给予阿奇霉素50 mg/（kg.d）和100 mg/（kg.d）灌服。在末次雾化吸入OVA后,经动物呼吸机测定豚鼠呼吸道压力。用支气管肺泡灌洗液（BALF）和肺组织病理分析的方法检测肺组织炎症;用免疫组织化学的方法检测NF-κB亚单位P65在细胞核内的表达。结果肺组织病理切片显示C、D、E组肺组织炎症细胞浸润的范围较B组明显减小。B组BALF中白细胞总数为（72.25±5.73）×108/L,C组为（41.93±4.05）×108/L,D组为（12.16±1.53）×108/L,E组为（10.31±2.61）×108/L,组间比较差异有高度统计学意义（P〈0.01）。C组肺组织单位面积内细胞核表达NF-κB阳性的细胞数为（2.81±0.15）×103/mm2,D组为（1.40±0.07）×103/mm2,E组为（1.13±0.12）×103/mm2,B组为（4.32±0.16）×103/mm2,组间比较差异有高度统计学意义（P〈0.01）。结论阿奇霉素干预组BALF中白细胞总数较模型组降低,细胞核表达NF-κB阳性的细胞数明显减少,证明阿奇霉素可明显减轻哮喘引起的气道炎症。

Effect of Azithromycin on expression of nuclear factor-kappa B in asthmatic guineapig

Objective To investigate the effects of the different dose of Azithromycin(AZM) on the expression of nuclear factor-kappa B(NF-κB) in the asthma guinea pigs.Methods 40 guinea pigs were randomly divided into five groups,which included normal control group(group A,n = 8),asthmatic model group(group B,n = 8),low dose AZM treatment group(group C,n = 8),moderate dose AZM treatment group(group D,n = 8),high dose AZM treatment group(group E,n = 8).After ovalbumin irritated,ovalbumin was inhaled to make asthma animal model.Azithromycin 25 mg/(kg·d) was given to group C,Azithromycin 50 mg/(kg·d),100 mg/(kg·d) were given to group D and E.After the last atomization inhalation,the airway pressure was detected by the animal respirator.Bronchoalveolar lavage fluid(BLAF) and lung tissue pathological analysis were used to describe lung tissue inflammation.The expression of NF-κB subunit P65 in cell nucleus of lung tissue was measured by immunohistochemistry.Results Lung tissue pathological analysis region indicated that the severity of allergic inflammation in group C,D,E was reduced than that in group B.The number of total WBC in BALF of group B was(72.25±5.73)×108/L,which was(41.93±4.05) ×108/L in group C,(12.16±1.53) ×108/L in group D,(10.31±2.61) ×108/L in group E,the differences were statistically significant(P < 0.01).The positive cell count of NF-кB expressed in the lung tissue of group C was(2.81±0.15) ×103/mm2,which of group D was(1.40±0.07) ×103/mm2,group E was(1.10±0.12) ×103/mm2,group B was(4.32±0.16) ×103/mm2,the differences were statistically significant(P < 0.01).Conclusion The number of total WBC in BALF and the expression of NF-κB in nucleus of AZM intervention group significantly reduced compared with those of the model group,which indicates that Azithromycin can significantly reduce the airway inflammation caused by asthma.