没药的质量分析与评价

目的建立客观评价没药药材质量的分析方法,对收集的不同批次没药药材的质量进行分析与综合评价,为其质量控制提供参考。方法采用GC-MS法对没药中挥发性成分进行分析与鉴定;采用HPLC-PDA法分析没药中非挥发性成分;并采用相似度软件和SPSS 16.0软件进行相似度评价和主成分分析。结果 GC-MS法分析鉴定了没药中28个化学成分,建立了不同批次没药药材非挥发性成分的HPLC特征图谱;两类指纹图谱相似度分析结果均表明,收集于不同批次的没药药材相似性较差,其中收集于海南和广东的相似度较高;主成分分析结果表明倍半萜烯类成分、有机酸酯类成分和二萜酸类成分对药材品质起到主导作用。综合分析提示,收集于海南药材公司的没药药材质量较佳。结论所建立的质量分析方法为没药质量控制提供了科学依据。

Quality analysis and evaluation of myrrh

Objective To establish the methods for the quality evaluation of Commiphora myrrha in different batches and provide the reference for quality control.Methods GC-MS and HPLC-PDA were used to analyze the volatile and non-volatile components,respectively.Similarity and principle components analysis(PCA) were carried out.Results Twenty-eight compounds were identified by GC-MS from C.myrrha and the HPLC fingerprint of non-volatile components was established.Results of similarity analysis showed that C.myrrha in different batches was different from each other except for batches from Hainan and Guangdong with high similarity.PCA demonstrated that sesquiterpenes,organic acid ester,and diterpenic acids played a leading role in the quality of C.myrrha.All results revealed that C.myrrha from Hainan had high qualities.Conclusion The established method could provide the guidance for quality control of C.myrrha.