ATPR对ECA-109、PANC-1、HeLa细胞增殖及分化的影响

背景与目的:由于全反式维甲酸(all-trans retinoic acid,ATRA)治疗急性早幼粒细胞白血病(acute promyelocytic leukemia,APL)存在一些不良反应,使得其进一步的临床应用受到了限制。目前,寻找具有更好疗效的维甲酸衍生物已成为研究热点。该研究旨在观察新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(4-amino-2-trifluoromethyl-phenyl retinate,ATPR)对食管癌细胞株ECA-109、胰腺癌细胞株PANC-1、宫颈癌细胞株HeLa生长和分化的影响。方法:采用MTT法检测细胞的生长抑制率;流式细胞仪测定细胞周期的变化;酶联免疫吸符实验(ELISA)检测食管癌细胞分化指标鳞状上皮癌相关抗原SCC-Ag活性、胰腺癌细胞标志物CA199和宫颈癌细胞标志物CA125水平。结果:ATPR能够抑制肿瘤细胞增殖;并使G0/G1期细胞表达量增加,S期细胞表达量减少,细胞阻滞在G0/G1期;ECA-109中SCC-Ag活性下降,PANC-1中CA199和HeLa中CA125水平下降。结论:ATPR对上述3种实体瘤细胞株具有不同程度的诱导分化作用。

Effects of a new retinoic acid derivative ATPR on the proliferation and differentiation of ECA-109,PANC-1 and HeLa tumor cells

Background and purpose:Due to some adverse reactions occurs when acute promyelocytic leukemia(APL) treated with all-trans retinoic acid(ATRA),which limited its further clinical application.At present,looking for a better efficacy of retinoic acid derivatives has become a hot topic.Therefore our study aimed to find the effects of 4-amino-2-trifluoromethyl-phenyl retinate(ATPR) on the proliferation and differentiation of human esophageal cancer cells ECA-109,human pancreatic cancer cells PANC-1 and human cervical cancer cells HeLa.Methods:Solid tumor cells were cultured in vitro and treated with ATPR of different concentrations.The proliferation was studied with MTT test.The cell cycle was analyzed by flow cytometry.SCC-Ag,CA199 and CA125 were measured by ELISA.Results:The growth of solid tumor cells treated with ATPR was inhibited in a dose-dependent manner;G0/G1 phase cells were significantly increased while S phase cells were decreased with the elevation of ATPR concentration,Cell cycle progression was blocked in the G0/G1 phase;SCC-Ag of ECA-109 suppressant;CA199 of PANC-1 and CA125 of HeLa decreased.Conclusion:ATPR could induce differentiation of solid tumor cells in different degrees.