| 非酒精性脂肪性肝炎模型的建立 |
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| 引用本文： | 王建青,李俊,邹宇宏,鲁超,葛金芳,刘翔,吴强.非酒精性脂肪性肝炎模型的建立[J].安徽医科大学学报,2006,41(1):61-63. |
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| 作者姓名： | 王建青 李俊 邹宇宏 鲁超 葛金芳 刘翔 吴强 |
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| 作者单位： | 安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032;安徽医科大学药学院,合肥,230032 |
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| 基金项目： | 安徽省人才开发资金资助(编号:2004Z030) |
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| 摘 要： | 目的建立大鼠非酒精性脂肪性肝炎（NASH）模型。方法将♂SD大鼠，随机分为正常组（n=15）和模型组（n=15）。两组动物均给以普通饲料，模型组每天ig高脂、高糖、高蛋白乳剂，剂量递增。实验持续6周后，检测血清ALT、AST、TC、TG、HDL-C、LDL-C及肝脏TC、TG，并行病理组织学检查。结果模型组大鼠血清ALT、AST、TC、HDL-C、LDL-C及肝脏TC、TG均显著增高；病理组织学检查提示模型组大鼠肝细胞呈现弥漫性脂肪变性伴肝小叶炎症坏死。结论利用改良乳剂灌胃的方法，成功复制出大鼠NASH模型。
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| 关 键 词： | 脂肪肝 疾病模型 动物 |
| 文章编号： | 1000-1492（2006）01-0061-03 |
| 收稿时间： | 2005-09-08 |
| 修稿时间： | 2005年9月8日 |
The establishment of nonalcoholic steatohepatitis animal model |
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| Wang Jianqing, Li Jun,Zou Yuhong, et al.The establishment of nonalcoholic steatohepatitis animal model[J].Acta Universitis Medicinalis Anhui,2006,41(1):61-63. |
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| Authors: | Wang Jianqing Li Jun Zou Yuhong |
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| Institution: | School of pharmacy, Anhui Medical University, Hefei 230032 |
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| Abstract: | Objective To establish a rat model of nonalcoholic steatohepatitis. Methods The model rats were fed with high fat, high sucrose and high protein emulsion via gavage (from 2.5~10 ml/kg, once a day) and standard rodent chow adlibitum for 6 weeks. Serum levels of ALT, AST, TC, TG, HDL, LDL, hepatic TC and TG content and histopathologic changes of liver were evaluated. Results The serum levels of ALT, AST, HDL, LDL, TC, TG, hepatic TC and TG content in model rats were significantly higher than those in normal rats. The histopathological analysis suggested that the model rats developed panlobular steatosis and inflammatory cell infiltration. Conclusion A NASH model is successfully induced in rats by feeding with high fat, high sucrose and high protein emulsion via gavage . |
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| Keywords: | fatty liver disease models animal |
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