Morphological characteristics and protein profile of isolated human decidual cells |
| |
| Authors: | K. Umapathysivam W.D.G. Haynes W.R. Jones H. Zola |
| |
| Affiliation: | 1. Department of Obstetrics and Gynecology, The Flinders University of South Australia, Bedford Park, South Australia, Australia;2. Department of Pathology, The Flinders University of South Australia, Bedford Park, South Australia, Australia;3. Department of Clinical Immunology, The Flinders University of South Australia, Bedford Park, South Australia, Australia |
| |
| Abstract: | Isolated decidual cells were prepared from human decidual tissue obtained during early pregnancy by digesting the tissue fragments with 0.1% collagenase solution. Morphological studies of the cells were carried out using morphometric and flow cytometric analysis while the protein profile was analysed by polyacrylamide gel electrophoresis and gel filtration column chromatography.An average of 90% cell viability was achieved and the results showed that decidual cells constitute up to 70% of cell number and 89% of cell area of the isolated decidual cell suspension. The presence of serum proteins in uterine tissue extracts is due to blood contamination. However, the similarities of the non-serum protein profiles in tissue and cellular extracts validates previous studies performed in uterine fluids and tissue extracts. Finally, at least one unique uterine protein appeared to be a sub-unit of a larger molecule. |
| |
| Keywords: | morphometry flow cytometry protein fractionation decidua |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
