| 他克莫司对骨髓间充质干细胞移植治疗大鼠脊髓损伤的影响 |
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| 引用本文： | 张建军,梁辉,盛学东,於永,刘泽朋,马景鑑. 他克莫司对骨髓间充质干细胞移植治疗大鼠脊髓损伤的影响[J]. 国际神经病学神经外科学杂志, 2008, 35(5): 398-402 |
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| 作者姓名： | 张建军 梁辉 盛学东 於永 刘泽朋 马景鑑 |
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| 作者单位： | 天津医科大学总医院神经外科,天津市,300052;天津医科大学总医院神经外科,天津市,300052;天津医科大学总医院神经外科,天津市,300052;天津医科大学总医院神经外科,天津市,300052;天津医科大学总医院神经外科,天津市,300052;天津医科大学总医院神经外科,天津市,300052 |
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| 基金项目： | 天津市应用基础研究项目 |
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| 摘 要： | 目的骨髓间充质干细胞(BMSC)移植同时应用他克莫司(FK506),观察两者对脊髓损伤后大鼠恢复的影响。方法用钳夹法制作大鼠急性脊髓损伤动物模型30只,随机分成3组。A组为单纯损伤组,B组为单纯骨髓干细胞移植组,C组为骨髓干细胞联合FK506组。于伤后1w、2w、4w、6w、8w采用BBB评分。8周后取材,行免疫组化及病理学检查。结果B、C两组大鼠后肢运动功能均有较明显恢复,C组较B组为快,两组统计学上有差异(P<0.05)。A组亦有所恢复,但程度较轻。A组切片,未见神经轴索通过,B、C组可见少量神经轴索样结构。免疫组化显示:A组NF200阳性细胞,GFAP阳性细胞均较B组C组少(P<0.05)。C组Nogo阳性神经元较A、B两组明显减少(P<0.05)。B组NF200阳性细胞,GFAP阳性细胞均较C组少(P<0.05)。结论骨髓间质干细胞移植对于后肢功能的恢复有促进作用,联合应用FK506有协同效果。
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| 关 键 词： | 脊髓损伤 骨髓问允质于细胞 FK506 功能恢复 |
Experimental Research on the Spine Regeneration Using BMSC and FK506 after SCI |
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| ZHANG Jian-jun,LIANG Hui,SHENG Xue-dong,YU Yong,LIU Ze-peng,MA Jing-jian. Experimental Research on the Spine Regeneration Using BMSC and FK506 after SCI[J]. Journal of International Neurology and Neurosurgery, 2008, 35(5): 398-402 |
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| Authors: | ZHANG Jian-jun LIANG Hui SHENG Xue-dong YU Yong LIU Ze-peng MA Jing-jian |
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| Affiliation: | ZHANG Jian-jun,LIANG Hui,SHENG Xue-dong,YU Yong,LIU Ze-peng,MA Jing-jian,Department of Neurosurgery,Tianjin Medical University General Hospital,Tianjin 300052 |
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| Abstract: | Objective To investigate the effect of bone mesenchymal stem cell(BMSC)and tacrolimus(FK506)on spinal cord injury(SCI).Methods The ring clamp SCI model of rat was constructed,30 rats were divided randomly into three groups,group A was single SCI group,group B was SCI group treated with BMSC injected,group C was SCI group treated just like group B but with FK506 which injected through abdominal part.In 1,2,4,6,8 weeks post-injury,all animals were evaluated of the hind limb behavior with BBB score test.The im... |
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| Keywords: | FK506 |
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