盐酸右美托咪定通过调控miR-223对子宫内膜癌细胞增殖和凋亡的影响

目的 探讨盐酸右美托咪定(Dex)对子宫内膜癌细胞AN3CA增殖和凋亡的影响及作用机制.方法 AN3CA 细胞分为对照组、不同浓度(1、2、5 μmol/L)Dex组、miR-223组、miR-NC组、5 μmol/L Dex+anti-miR-223组和5μmol/LDex+anti-miR-NC组.MTT法检测细胞...

Effect of dexmedetomidine hydrochloride on proliferation and apoptosis of endometrial cancer cells by regulating miR-223

Objective To investigate the effects and mechanism of dexmedetomidine hydrochloride(Dex)on proliferation and apoptosis of endometrial cancer cell line AN3CA.Methods AN3CA cells were divided into control group,Dex groups of different concentrations(1,2,5μmol/L correspongdingly),miR-223 group,miR-NC group,5μmol/L Dex+anti-miR-223 group,5μmol/L Dex+anti-miR-NC group.MTT was used to detect cell proliferation,flow cytometry was used to detect apoptosis,Western blot was used to detect expression levels of CyclinD1,P21,Bcl-2 and Bax protein,and RT-qPCR was used to detect expression level of miR-223.Results Compared with the control group,AN3CA cell activity,the protein levels of CyclinD1 and Bcl-2 decreased in Dex groups(P<0.05),while the apoptosis rate,the protein levels of P21 and Bax,and miR-223 levels increased(P<0.05).Compared with the miR-NC group,AN3CA cell activity,the protein levels of CyclinD1 and Bcl-2 decreased in the miR-223 group(P<0.05),while the apoptosis rate and the protein levels of P21 and Bax increased(P<0.05).Compared with the 5μmol/L Dex+anti-NC group,AN3CA cell activity and the protein levels of CyclinD1 and Bcl-2 increased in the 5μmol/L Dex+anti-miR-223 group(P<0.05),while the apoptosis rate and the protein levels of P21 and Bax decreased(P<0.05).Conclusion Dex may inhibit the proliferation of endometrial cancer cell AN3CA and promote its apoptosis by up-regulating miR-223.