基因工程双价抗体在肿瘤中的研究进展

基因工程抗体技术的发展加速了单链抗体的应用,但其稳定性差，亲和力低，功能单一，体内清除过快等特点影响了它的广泛应用。双价抗体作为一种新型小分子抗体，具有双价的结合位点,能够使抗原分子上的两个表位交联或使两个分子连接，可以模拟完整的单克隆抗体的抗原抗体反应，其构建方法有亮氨酸拉链法、利用部分抗体恒定区法、连接肽法、利用双聚化结构法、knobs into holes技术等，在乳腺癌、 直肠癌、淋巴瘤等的诊治方面均有很好的应用价值。

Development of genetically engineered bivalent antibodies in tumor

The development of genetically engineered antibodies technique accelerates the application of single chain antibody,but the characteristics including the poor stability,low affinity,single function,fast clearance affect their extensive application.Bivalent antibodies have two binding sites that can crosslink one antigen particle to another,or can allow antibodies to bind to multimeric antigen with great avidity,so called a new antibody.They cause a visible reaction with specific antigen just as monoclonal antibody.They can be constructed by means of leuzine zipper,constant region of antibodies,limker,etc and have a great potential in therapeutic and diagnostic aspects of breast cancer,colorectal cancer,lymphoma,and so on.