不同针刺刺激量对寒凝证类痛经大鼠子宫收缩强度及内分泌相关分子受体基因表达的影响?

目的：观察不同刺激量针刺三阴交对寒凝证类痛经大鼠子宫张力及内分泌相关分子受体基因表达的影响，探寻不同针刺刺激量与靶器官效应之间的关系。方法将动情期间雌性大鼠随机分为盐水组、寒凝证类痛经大鼠模型(模型组)、期望“得气”组(粗针深刺行针的治疗方法)、期望“不得气”组(细针浅刺不行针的治疗方法)；期望“得气”组或期望“不得气”组均在成功制备模型基础上给予治疗，并于针刺进行时观察20 min内在体大鼠子宫收缩强度及大鼠子宫组织前列腺素EP2受体mRNA (PGEP2mRNA)、前列腺素F2α受体mRNA(PGF2αmRNA)表达量。结果子宫收缩强度检测方面，与模型组比较，期望“得气”组显著减少了子宫收缩波个数(P<0.01)，显著降低子宫活动度(P<0.05)。子宫PGEP2 mRNA及PGF2αmRNA检测方面，与模型组比较，期望“得气”组及期望“不得气”组 PGF2αmRNA表达显著降低( P<0.01)。结论不同刺激量针刺三阴交穴均能缓解寒凝证类痛经大鼠子宫收缩强度及降低子宫内分泌相关分子受体基因表达，使用粗针深刺加行针的治疗效果优于细针浅刺不行针，认为粗针深刺加行针的刺激量更能达到针灸“得气”的状态。

Effects of acupuncture with different stimuli intensity on uterine contraction and expression of endocrine-related molecular receptor genes in rats with dysmenorrhea of cold coagulation pattern

Objective To evaluate the effects of acupuncture with different stimulation intensity at Sanyinjiao (SP6) on uterine contraction and expression of endocrine-related molecular receptor gene in rats with dysmenorrhea of cold coagulation pattern and to explore whether there is association between“deqi ( qi arrival)” and stimulus intensity. Methods Diestrus female rats were randomly divided into 4 groups, in-cluding saline control group, model control group, deqi group ( with strong stimulus) and No deqi group ( with weak stimulus) . The levels of uterine contraction was recorded during 20 mins acupuncture treat-
ment. The expression of PGF2α mRNA was measured. Results Compared with the model control group, the uterine contraction wave number and uterine activity were significantly reduced in deqi group. The ex-pressions of PGF2αmRNA in both deqi and No deqi group were also reduced compared with model group. Conclusion Our finding indicates that using thick needles, deep insertion with hand-manipulation seems more effective and could achieve desired level of “deqi” in dysmenorrhea rats.