蒙药复方森登-4体内外化学成分分析

目的：建立蒙药复方森登-4体内外特征图谱,归属并鉴定其体外化学成分及体内吸收入血成分。方法取SD大鼠,建立类风湿性关节炎动物模型,灌胃蒙药复方森登-4及其各单味药有效组分,分离制备含药血清,HPLC法测定,建立蒙药复方森登-4体外及含药血清HPLC特征图谱,分析蒙药复方森登-4、各单味药体外及其大鼠含药血清特征图谱,比对、归属并鉴定蒙药复方森登-4体外化学成分及口服蒙药复方森登-4后的大鼠吸收入血成分。结果分析蒙药复方森登-4体外HPLC特征图谱,发现了共47个峰；蒙药复方森登-4灌胃给药后出现了14个入血成分,9个为体外成分直接入血,另外5个推测为新生代谢产物,2号峰来源于文冠木,5、6、7号峰来源于栀子,1、8、10号峰来源于诃子,3号峰来源复杂,均来源于文冠木、川楝子、诃子,4、13号峰来源于全方,9号峰来源于文冠木及川楝子,11、12、14号峰来源于文冠木及诃子并鉴定出7个蒙药复方森登-4成分。结论血中成分及代谢产物可能为蒙药复方森登-4体内直接作用的有效成分,分别来源于蒙药复方森登-4的各单味药。

Analyzing chemical composition of Mongolian compound Sengdeng-4 in vivo and in vitro

Objective To establish the characteristic chromatograms of Mongolian compound Sengdeng-4 in vivo and in vitro, analyze and identify its chemical components in vitro and serum. Methods After the model of rheumatoid arthritis was established, SD rats were orally administered Mongolian compound Sengdeng-4 and each single ingredient respectively. The medicinal serum were separated and measured with HPLC. The HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 in vitro and medicinal serum were identified. The characteristic chromatograms of Mongolian compound Sengdeng-4, single ingredient in vitro and medicinal serum were analyzed, and the chemical composition of Mongolian compound Sengdeng-4 in vitro, original crude medicinal in blood were identified and compared. Results The results of HPLC characteristic chromatograms of Mongolian compound Sengdeng-4 showed that 47 peaks were identified. There were 14 chemical compositions absorbed into blood, among them 9 were original ingredients and 5 were metabolites. The second peak was from Wenguanmu; the fifth, sixth, and seventh peaks were from Zhizi; the first, eighth, and tenth peaks were from Hezi. The third peak was from Wenguanmu, Chuanlianzi and Hezi. The fourth and thirteenth peaks were from Mongolian compound Sengdeng-4. The ninth peak was from Wenguanmu and Chuanlianzi. The eleventh, twelfth
and fourteenth peaks were from Wenguanmu and Hezi. 7 components of Mongolian compound Sengdeng-4 were identified. Conclusion The chemical compositions and metabolites in blood may be the active ingredients with direct actions in vivo, which come from the ingredients of Mongolian compound Sengdeng-4 .