脂质体Geneshuttle20对STAT6反义核酸转染小鼠脾淋巴细胞影响的研究

目的探讨阳离子脂质体Geneshuttle20对STAT6反义核酸在小鼠脾淋巴细胞摄入、分布的影响作用。方法采用阳离子脂质体介导STAT6反义核酸转染小鼠脾淋巴细胞,应用流式细胞仪、荧光显微镜分别观察反义核酸的细胞摄入和胞内分布。结果反义核苷酸与脂质体(W/W)为2∶4时,细胞摄入率可以达到67.7%,较单独加入反义核酸提高转染效率6倍,细胞内平均荧光强度最强,细胞核染色较深。结论Geneshuttle20提高了细胞对反义寡核苷酸的摄取,促使其进入细胞核内发挥作用。

The role of Geneshuttle20, a cationic liposome, in STAT6 antisense oligonucleotide transduction of murine spleen lymphocytes

Objective To study the role of a cationic liposome, Geneshuttle20, on the uptake and distribution of STAT6 antisense oligonucleotide in murine spleen lymphocytes. Methods The uptake and intracellular distribution of STAT6 antisense oligonucleotide mediated by Geneshuttle20 were detected by flow cytometry and fluorescence microscopy. Results Compared with antisense oligonucleotide alone, combination of antisense oligonucleotide and cationic liposome in 2:4 w/w ratio showed a 6-fold rise (67.7%) in lymphocyte uptake, with highest intensity of intracellular fluorescence and nuclear staining. Conclusion Geneshuttle20 significantly enhance the uptake of STAT6 antisense oligonucleotide by murine spleen lymphocytes and facilitated its intranuclear effects.