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| 黑素瘤抑制蛋白在转基因小鼠胚胎乳腺组织中的表达 | |
| 引用本文： | 谢渭芬,高勇,贺祥,李舰,朱(),陈伟忠,林勇. 黑素瘤抑制蛋白在转基因小鼠胚胎乳腺组织中的表达[J]. 第二军医大学学报, 2000, 21(1): 29-30,I002 |
| 作者姓名： | 谢渭芬 高勇 贺祥 李舰 朱() 陈伟忠 林勇 |
| 作者单位： | 1. 第二军医大学长征医院消化内科,上海,200032 2. 长征医院肿瘤科 3. 第二军医大学科研部 |
| 基金项目： | 国家“863”计划资助!项目（102－10－04－03） |
| 摘 要： | 目的：检测黑素瘤抑制蛋白（ＭＩＡ／ＣＤ－ＲＡＰ）在小鼠胚胎乳腺组织中的表达。方法：剖腹分离出１０．５－１６．５ｄ的ＭＩＡ－ＣＤ／ＲＡＰ启动子－半乳糖苷酶（２．２１ｌａｃＺ）转基因小鼠胚胎，利用Ｘ－ｇａｌ全胚胎染色观察转基因的组织和细胞染色，并以免疫组织化学染色检测内源性ＭＩＡ／ＣＤ－ＲＡＰ蛋白在胚胎乳腺组织叫的表达。结果：ＭＩＡ／ＣＤ－ＲＡＰ在孕期１１．５ｄ的小鼠胚胎乳腺上皮细胞中开始表达，至１３ |
| 关 键 词： | 黑素瘤抑制蛋白 小鼠 转基因 乳腺癌 |
| 文章编号： | 0258-879X(2000)01-0029-02 |
Expression of melanoma inhibitory protein in mammary buds of transgenic mice |
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| XIE Wei-Fen,GAO Yong,HE Xiang,LI Jian,ZHU Liang,CHEN Wei-Zhong,LIN Yong. Expression of melanoma inhibitory protein in mammary buds of transgenic mice[J]. Former Academic Journal of Second Military Medical University, 2000, 21(1): 29-30,I002 | |
| Authors: | XIE Wei-Fen GAO Yong HE Xiang LI Jian ZHU Liang CHEN Wei-Zhong LIN Yong |
| Affiliation: | XIE Wei-Fen(Department of Gastroenterology, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)LI Jian(Department of Gastroenterology, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)ZHU Liang(Department of Gastroenterology, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)CHEN Wei-Zhong(Department of Gastroenterology, Changzheng Hospital, Second Military Medical University,Shanghai 200003, China)LIN Yong(Department of Gastroenterology, Changzheng Hospital, Second Military MedicalUniversity, Shanghai 200003, China)GAO Yong(Department of Oncology, Changzheng Hospital)HE Xiang(Department of Science and Research, Second Military Medical University) |
| Abstract: | Objective: To investigate the expression of melanoma inhibitory protein (MIA) in mammary buds of transgenic mice. Methods: Transgenic embryos were collected from day 10.5 to 16.5 by Cesarean section. The transgene expression was detected by whole mount staining with X gal. Immunohistochemistry was carried out to detect endogenous MIA/CD RAP expression in mammary buds. Results: MIA/CD RAP expression in mammary epithelial cells began on day 11.5 p.c. and continued throughout day 15.5 p.c., with the strongest expression observed on day 13.5 p.c. Conclusion: MIA/CD RAP may play an important role in the early organogenesis of mammary gland and carcinogenesis of breast cancer. |
| Keywords: | melanoma inhibitory protein cartilage derived retinoic acid sensitive protein mice transgenic mammary gland |
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